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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Induction of lignocellulose-degrading enzymes in Neurospora crassa by cellodextrins
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Induction of lignocellulose-degrading enzymes in Neurospora crassa by cellodextrins

机译：纤维糊精诱导神经孢菌中木质纤维素降解酶

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摘要

Neurospora crassa colonizes burnt grasslands in the wild and metabolizes both cellulose and hemicellulose from plant cell walls. When switched from a favored carbon source such as sucrose to cellulose, N. crassa dramatically upregulates expression and secretion of a wide variety of genes encoding lignocellulolytic enzymes. However, the means by which N. crassa and other filamentous fungi sense the presence of cellulose in the environment remains unclear. Here, we show that an N. crassa mutant carrying deletions of two genes encoding extracellular p-glucosidase enzymes and one intracellular p-glucosidase lacks p-glucosidase activity, but efficiently induces cellulase gene expression in the presence of cel-lobiose, cellotriose, or cellotetraose as a sole carbon source. These data indicate that cellobiose, or a modified version of cellobiose, functions as an inducer of lignocellulolytic gene expression in, N. crassa. Furthermore, the inclusion of a deletion of the catabolite repressor gene, cre-1, in the triple p-glucosidase mutant resulted in a strain that produces higher concentrations of secreted active cel-lulases on cellobiose. Thus, the ability to induce cellulase gene expression using a common and soluble carbon source simplifies enzyme production and characterization, which could be applied to other cellulolytic filamentous fungi.
机译：芥菜神经孢菌在野生的定居草原上定居，并从植物细胞壁中代谢纤维素和半纤维素。当从偏爱的碳源（例如蔗糖）转换为纤维素时，猪笼草会显着上调编码木质纤维素分解酶的多种基因的表达和分泌。但是，尚不能鉴定克雷萨氏菌和其他丝状真菌在环境中存在纤维素的方法。在这里，我们显示携带两个编码胞外p-葡萄糖苷酶的基因缺失和一个胞内p-葡萄糖苷酶的crassa突变体缺乏p-葡萄糖苷酶活性，但是在存在胞果糖，纤维三糖或纤维素时有效诱导纤维素酶基因表达。纤维四糖是唯一的碳源。这些数据表明，纤维二糖或纤维二糖的修饰形式，在猪笼草中起木质纤维素分解基因表达的诱导作用。此外，在三重p-葡糖苷酶突变体中包括分解代谢物阻遏物基因cre-1的缺失，导致菌株在纤维二糖上产生更高浓度的分泌的活性纤维素。因此，使用常见的可溶性碳源诱导纤维素酶基因表达的能力简化了酶的产生和表征，这可应用于其他纤维素分解丝状真菌。

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    Elizabeth A. Znameroski; Samuel T. Coradetti; Christine M. Roche; Jordan C. Tsai; Anthony T. lavarone; Jamie H. D. Cate; N. Louise Glass;

  • 作者单位

    Departments of Molecular and Cell Biology, University of California, Berkeley, CA 94720;

    Plant and Microbial Biology, University of California, Berkeley, CA 94720;

    Chemical and Biomolecular Engineering, University of California, Berkeley, CA 94720;

    Departments of Molecular and Cell Biology, University of California, Berkeley, CA 94720,Chemistry, University of California, Berkeley, CA 94720;

    California Institute for Quantitative Biosciences (QB3), University of California, Berkeley, CA 94720;

    Departments of Molecular and Cell Biology, University of California, Berkeley, CA 94720,Chemical and Biomolecular Engineering, University of California, Berkeley, CA 94720,Chemistry, University of California, Berkeley, CA 94720;

    Plant and Microbial Biology, University of California, Berkeley, CA 94720;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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