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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Tubulin homolog TubZ in a phage-encoded partition system
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Tubulin homolog TubZ in a phage-encoded partition system

机译：噬菌体编码分区系统中的微管蛋白同源物TubZ

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Partition systems are responsible for the process whereby large and essential plasmids are accurately positioned to daughter cells during bacterial division. They are typically made of three components: a centromere-like DNA zone, an adaptor protein, and an assembling protein that is either a Walker-box ATPase (type I) or an actin-like ATPase (type II). A recently described type III segregation system has a tubulin/FtsZ-like protein, called TubZ, for plasmid movement. Here, we present the 2.3 A structure and dynamic assembly of a TubZ tubulin homolog from a bacteriophage and unravel the Clostridium botulinum phage c-st type III partition system. Using biochemical and biophysical approaches, we prove that a gene upstream from tubZ encodes the partner TubR and localize the centromeric region (tubS), both of which are essential for anchoring phage DNA to the motile TubZ filaments. Finally, we describe a conserved fourth component, TubY, which modulates the TubZ-R-S complex interaction.
机译：分区系统负责在细菌分裂过程中将大而重要的质粒准确定位到子细胞的过程。它们通常由三部分组成：着丝粒样DNA区域，衔接蛋白和组装蛋白，它们是Walker-box ATPase（I型）或肌动蛋白样ATPase（II型）。最近描述的III型分离系统具有微管蛋白/ FtsZ样蛋白，称为TubZ，用于质粒移动。在这里，我们目前从细菌噬菌体的TubZ微管蛋白同源物的2.3 A结构和动态组装，并解开了肉毒梭菌噬菌体c-st III型分配系统。使用生化和生物物理方法，我们证明了tubZ上游的基因编码伴侣TubR并定位着丝粒区域（tubS），这两者对于将噬菌体DNA锚定到活动的TubZ细丝都是必不可少的。最后，我们描述了一个保守的第四成分TubY，它调节TubZ-R-S复杂的相互作用。

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