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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Asymmetrically expressed axin required for anterior development in Tribolium
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Asymmetrically expressed axin required for anterior development in Tribolium

机译：Tribolium前发展所需的不对称表达的毒素

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摘要

Canonical Wnt signaling has been implicated in an AP axis polarizing mechanism in most animals, despite limited evidence from arthropods. In the long-germ insect, Drosophila, Wnt signaling is not required for global AP patterning, but in short-germ insects including Tribolium castaneum, loss of Wnt signaling affects development of segments in the growth zone but not those defined in the blastoderm. To determine the effects of ectopic Wnt signaling, we analyzed the expression and function of axin, which encodes a highly conserved negative regulator of the pathway. We found Tc-axin transcripts maternally localized to the anterior pole in freshly laid eggs. Expression spread toward the posterior pole during the early cleavage stages, becoming ubiquitous by the time the germ rudiment formed. Tc-axin RNAi produced progeny phenotypes that ranged from mildly affected embryos with cuticles displaying a graded loss of anterior structures, to defective embryos that condensed at the posterior pole in the absence of serosa. Altered expression domains of several blastodermal markers indicated anterior expansion of posterior fates. Analysis of other canonical Wnt pathway components and the expansion of Tc-caudal expression, a Wnt target, suggest that the effects of Tc-axin depletion are mediated through this pathway and that Wnt signaling must be inhibited for proper anterior development in Tribolium. These studies provide unique evidence that canonical Wnt signaling must be carefully regulated along the AP axis in an arthropod, and support an ancestral role for Wnt activity in defining AP polarity and patterning in metazoan development.
机译：尽管节肢动物的证据有限，但在大多数动物中，规范的Wnt信号传导与AP轴极化机制有关。在长果蝇的果蝇中，Wnt信号不是整体AP模式所必需的，但是在包括Tribolium castaneum在内的短芽昆虫中，Wnt信号的缺失会影响生长区中节段的发育，但不会影响胚盘中的节段。为了确定异位Wnt信号转导的影响，我们分析了axin的表达和功能，它编码了该途径的高度保守的负调节剂。我们发现Tc-axin转录本位于新鲜产卵的母体中，位于前极。在早期卵裂期，表达向后极扩散，在胚芽形成时就变得无处不在。 Tc-axin RNAi产生的后代表型从轻度受累的表皮显示前部结构逐渐丧失的表皮到不存在浆膜的在后极凝聚的有缺陷的胚胎。几种胚胚标记物表达域的改变表明后缘的前向扩展。对其他经典Wnt途径成分和Tc尾部表达（Wnt靶标）的扩展的分析表明，Tc毒素消耗的影响是通过该途径介导的，并且必须抑制Wnt信号传导才能在Tribolium中适当地向前发展。这些研究提供了独特的证据，即规范的Wnt信号必须在节肢动物中沿着AP轴仔细调节，并支持Wnt活性在定义AP极性和后生动物发育中的祖先作用。

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    Jinping Fu; Nico Posnien; Renata Bolognesi; Tamara D. Fischer; Parker Rayl; Georg Oberhofer; Peter Kitzmann; Susan J. Brown; Gregor Bucher;

  • 作者单位

    Division of Biology, Kansas State University, Manhattan, KS 66502;

    Department of Developmental Biology, Johann Friedrich Blumenbach Institute of Zoology and Anthropology, Georg-August-University, 37077 Goettingen, Germany, Department of Biological and Medical Sciences, Faculty of Health and Life Sciences, Oxford Brookes University, Oxford OX3 OBP, United Kingdom;

    Division of Biology, Kansas State University, Manhattan, KS 66502, Monsanto Co., St. Louis, MO, 63017;

    Division of Biology, Kansas State University, Manhattan, KS 66502;

    Division of Biology, Kansas State University, Manhattan, KS 66502;

    Department of Developmental Biology, Johann Friedrich Blumenbach Institute of Zoology and Anthropology, Georg-August-University, 37077 Goettingen, Germany;

    Department of Developmental Biology, Johann Friedrich Blumenbach Institute of Zoology and Anthropology, Georg-August-University, 37077 Goettingen, Germany;

    Division of Biology, Kansas State University, Manhattan, KS 66502;

    Department of Developmental Biology, Johann Friedrich Blumenbach Institute of Zoology and Anthropology, Georg-August-University, 37077 Goettingen, Germany;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    A-P axis patterning; short-germ segmentation; anterior patterning; maternal determination;

    机译：A-P轴构图;短期细菌细分;前部图案产妇决心;

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