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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Cumulative lesioning of respiratory interneurons disrupts and precludes motor rhythms in vitro
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Cumulative lesioning of respiratory interneurons disrupts and precludes motor rhythms in vitro

机译：呼吸中枢神经的累积损伤可在体外破坏并阻止运动节律

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摘要

How brain functions degenerate in the face of progressive cell loss is an important issue that pertains to neurodegenerative diseases and basic properties of neural networks. We developed an automated system that uses two-photon microscopy to detect rhythmic neurons from calcium activity, and then individually laser ablates the targets while monitoring network function in real time. We applied this system to the mammalian respiratory oscillator located in the pre-Boetzinger Complex (preBoetC) of the ventral medulla, which spontaneously generates breathing-related motor activity in vitro. Here, we show that cumulatively deleting preBotC neurons progressively decreases respiratory frequency and the amplitude of motor output. On average, the deletion of 120 + 45 neurons stopped spontaneous respiratory rhythm, and our data suggest ≈82% of the rhythm-generating neurons remain unlesioned. Cumulative ablations in other medullary respiratory regions did not affect frequency but diminished the amplitude of motor output to a lesser degree. These results suggest that the preBoetC can sustain insults that destroy no more than ≈18% of its constituent interneurons, which may have implications for the onset of respiratory pathologies in disease states.
机译：面对进行性细胞丢失，大脑功能如何退化是一个重要问题，与神经退行性疾病和神经网络的基本特性有关。我们开发了一种自动化系统，该系统使用双光子显微镜从钙活动中检测有节奏的神经元，然后分别激光消融靶标，同时实时监控网络功能。我们将此系统应用于位于腹髓的前Boetzinger复合体（preBoetC）中的哺乳动物呼吸振荡器，该振荡器在体外自发产生与呼吸有关的运动活动。在这里，我们显示出累积删除preBotC神经元会逐渐降低呼吸频率和运动输出的幅度。平均而言，删除120 + 45个神经元会停止自发性呼吸节律，我们的数据表明，约有82％的产生节奏的神经元未受损。其他延髓性呼吸区域的累积消融不会影响频率，但会在较小程度上降低运动输出的幅度。这些结果表明，preBoetC可以承受破坏不超过其构成中间神经元的≈18％的侮辱，这可能与疾病状态下呼吸道疾病的发作有关。

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  • 作者

    John A. Hayes; Xueying Wang; Christopher A. Del Negro;

  • 作者单位

    Departments of Applied Science and College of William and Mary, Williamsburg, VA 23187-8795, Departments of Biology, College of William and Mary, Williamsburg, VA 23187-8795 Neurobiologie et Developpement, Institut de Neurobiologie Alfred Fes- sard, Centre National de la Recherche Scientifique, 91198 Gif-sur-Yvette, France;

    Departments of Applied Science and College of William and Mary, Williamsburg, VA 23187-8795;

    Departments of Applied Science and College of William and Mary, Williamsburg, VA 23187-8795;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    central pattern generator; calcium imaging; brainstem;

    机译：中央模式发生器;钙成像脑干;

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