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A mouse model for HIV-1 entry

机译:HIV-1进入的小鼠模型

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摘要

Passive transfer of neutralizing antibodies against HIV-1 can prevent infection in macaques and seems to delay HIV-1 rebound in humans. Anti-HIV antibodies are therefore of great interest for vaccine design. However, the basis for their in vivo activity has been difficult to evaluate systematically because of a paucity of small animal models for HIV infection. Here we report a genetically humanized mouse model that incorporates a luciferase reporter for rapid quantitation of HIV entry. An antibody's ability to block viral entry in this in vivo model is a function of its bioavailability, direct neutralizing activity, and effector functions.
机译:被动转移抗HIV-1的中和抗体可以预防猕猴感染,并且似乎可以延迟人类HIV-1的反弹。因此,抗HIV抗体对于疫苗设计非常重要。但是,由于缺乏用于感染HIV的小动物模型,因此难以系统评估其体内活性的基础。在这里,我们报告了一种遗传人性化的小鼠模型,该模型结合了荧光素酶报道基因,可快速定量HIV进入。抗体在此体内模型中阻止病毒进入的能力是其生物利用度,直接中和活性和效应子功能的函数。

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    Laboratory of Molecular Immunology, Laboratory of Virology and Infectious Diseases, The Rockefeller University, New York, NY 10065,Department of Biology, Chemistry, and Pharmacy, Freie Universitat Berlin, D14195 Berlin, Germany;

    Laboratory of Molecular Immunology, Laboratory of Virology and Infectious Diseases, The Rockefeller University, New York, NY 10065,Medizinische Fakultat, Westfalische Wilhelms-Universitaet Muenster, D48149 Muenster, Germany;

    Laboratory of Molecular Genetics and Immunology, Laboratory of Virology and Infectious Diseases, The Rockefeller University, New York, NY 10065;

    Center for the Study of Hepatitis C, Laboratory of Virology and Infectious Diseases, The Rockefeller University, New York, NY 10065;

    Laboratory of Molecular Immunology, Laboratory of Virology and Infectious Diseases, The Rockefeller University, New York, NY 10065;

    Division of Biology, California Institute of Technology, Pasadena, CA 91125;

    Division of Viral Pathogenesis, Beth Israel Deaconess Medical Center, Boston, MA 02215;

    Division of Biology, California Institute of Technology, Pasadena, CA 91125,Howard Hughes Medical Institute, California Institute of Technology, Pasadena, CA 91125;

    Laboratory of Molecular Genetics and Immunology, Laboratory of Virology and Infectious Diseases, The Rockefeller University, New York, NY 10065;

    Center for the Study of Hepatitis C, Laboratory of Virology and Infectious Diseases, The Rockefeller University, New York, NY 10065;

    Laboratory of Molecular Immunology, Laboratory of Virology and Infectious Diseases, The Rockefeller University, New York, NY 10065,Laboratory of Molecular Immunology, Laboratory of Virology and Infectious Diseases, The Rockefeller University, New York, NY 10065;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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