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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Transcriptional repressor PRR5 directly regulates clock-output pathways
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Transcriptional repressor PRR5 directly regulates clock-output pathways

机译：转录阻遏物PRR5直接调节时钟输出途径

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摘要

The circadian clock is an endogenous time-keeping mechanism that enables organisms to adapt to external daily cycles. The clock coordinates biological activities with these cycles, mainly through genome-wide gene expression. However, the exact mechanism underlying regulation of circadian gene expression is poorly understood. Here we demonstrated that an Arabidopsis PSEUDO-RESPONSE REGULATOR 5 (PRR5), which acts in the clock genetic circuit, directly regulates expression timing of key transcription factors involved in clock-output pathways. A transient expression assay and ChlP-quantitative PCR assay using mutated PRR5 indicated that PRR5 associates with target DNA through binding at the CCT motif in vivo. ChIP followed by deep sequencing coupled with genome-wide expression profiling revealed the direct-target genes of PRR5. PRR5 direct-targets include genes encoding transcription factors involved in flowering-time regulation, hypocotyl elongation, and cold-stress responses. PRR5-target gene expression followed a circadian rhythm pattern with low, basal expression from noon until midnight, when PRR9, PRR7, and PRR5 were expressed. ChlP-quantitative PCR assays indicated that PRR7 and PRR9 bind to the direct-targets of PRR5. Genome-wide expression profiling using a prr9 prr7 prrS triple mutant suggests that PRR5, PRR7, and PRR9 repress these targets. Taken together, our results illustrate a genetic network in which PRR5, PRR7, and PRR9 directly regulate expression timing of key transcription factors to coordinate physiological processes with daily cycles.
机译：昼夜节律时钟是一种内在的计时机制，使生物能够适应外部的日常循环。时钟主要通过全基因组基因表达来协调这些周期的生物活动。然而，对昼夜节律基因表达调控的确切机制了解甚少。在这里，我们证明了拟南芥PSEUDO-RESPONSE REGULATOR 5（PRR5）在时钟遗传电路中起作用，直接调节参与时钟输出途径的关键转录因子的表达时间。使用突变的PRR5的瞬时表达测定法和ChlP定量PCR测定法表明PRR5通过体内CCT基序的结合与靶DNA结合。 ChIP随后进行深度测序，再加上全基因组表达谱分析，揭示了PRR5的直接靶基因。 PRR5直接靶标包括编码与开花时间调控，下胚轴伸长和冷胁迫反应有关的转录因子的基因。从中午到午夜，当表达PRR9，PRR7和PRR5时，PRR5-靶基因的表达遵循昼夜节律模式，基础表达低。 ChlP定量PCR分析表明，PRR7和PRR9与PRR5的直接靶标结合。使用prr9 prr7 prrS三重突变体的全基因组表达谱分析表明PRR5，PRR7和PRR9抑制了这些靶标。两者合计，我们的结果说明了一个遗传网络，其中PRR5，PRR7和PRR9直接调节关键转录因子的表达时间，以协调生理过程与每日周期。

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  • 作者

    Norihito Nakamichi; Takatoshi Kiba; Mari Kamioka; Takamasa Suzuki; Takafumi Yamashino; Tetsuya Higashiyama; Hitoshi Sakakibara; Takeshi Mizuno;

  • 作者单位

    Institute for Advanced Research , Nagoya University, Furo-cho, Chikusa, Nagoya 464-8601, Japan,School of Agriculture, Nagoya University, Furo-cho, Chikusa, Nagoya 464-8601, Japan,Plant Productivity Systems Research Group, RIKEN Plant Science Center, Tsurumi, Yokohama 230-0045,Precursory Research for Embryonic Science and Technology, Japan Science and Technology Agency, Kawaguchi, Saitama 332-0022, Japan;

    Plant Productivity Systems Research Group, RIKEN Plant Science Center, Tsurumi, Yokohama 230-0045;

    School of Agriculture, Nagoya University, Furo-cho, Chikusa, Nagoya 464-8601, Japan;

    Exploratory Research for Advanced Technology Higashiyama Live-Holonics Project, Nagoya University, Furo-cho, Chikusa, Nagoya 464-8602, Japan,Division of Biological Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa, Nagoya 464-8602, Japan;

    School of Agriculture, Nagoya University, Furo-cho, Chikusa, Nagoya 464-8601, Japan;

    Exploratory Research for Advanced Technology Higashiyama Live-Holonics Project, Nagoya University, Furo-cho, Chikusa, Nagoya 464-8602, Japan,Division of Biological Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa, Nagoya 464-8602, Japan;

    Plant Productivity Systems Research Group, RIKEN Plant Science Center, Tsurumi, Yokohama 230-0045;

    School of Agriculture, Nagoya University, Furo-cho, Chikusa, Nagoya 464-8601, Japan;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    ChIP-seq; plant;

    机译：芯片序列厂;

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