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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Functional eukaryotic nuclear localization signals are widespread in terminal proteins of bacteriophages
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Functional eukaryotic nuclear localization signals are widespread in terminal proteins of bacteriophages

机译：功能性真核核定位信号广泛分布在噬菌体的末端蛋白中

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摘要

A number of prokaryotic proteins have been shown to contain nuclear localization signals (NLSs), although its biological role remains sometimes unclear. Terminal proteins (TPs) of bacteriophages prime DNA replication and become covalently linked to the genome ends. We predicted NLSs within the TPs of bacteriophages from diverse families and hosts and, indeed, the TPs of Φ29, Nf, PRD1, Bam35, and Cp-1, out of seven TPs tested, were found to localize to the nucleus when expressed in mammalian cells. Detailed analysis of Φ29 TP led us to identify a bona fide NLS within residues 1-37. Importantly, gene delivery into the eukaryotic nucleus is enhanced by the presence of Φ29 TP attached to the 5' DNA ends. These findings show a common feature of TPs from diverse bacteriophages targeting the eukaryotic nucleus and suggest a possible common function by facilitating the horizontal transfer of genes between prokaryotes and eukaryotes.
机译：已显示许多原核蛋白含有核定位信号（NLSs），尽管其生物学作用有时仍不清楚。噬菌体的末端蛋白（TPs）引发DNA复制，并与基因组末端共价连接。我们预测了来自不同家族和宿主的噬菌体TP内的NLS，实际上，在测试的7种TP中，Φ29，Nf，PRD1，Bam35和Cp-1的TP在哺乳动物中表达时均定位于细胞核。细胞。 Φ29TP的详细分析使我们在残基1-37中鉴定出了真正的NLS。重要的是，通过附着在5'DNA末端的Φ29TP可以增强基因向真核细胞内的传递。这些发现表明，靶向真核细胞的各种噬菌体中的TPs的共同特征，并通过促进原核生物和真核生物之间基因的水平转移，暗示了可能的共同功能。

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    Modesto Redrejo-Rodriguez; Daniel Munoz-Espin; Isabel Holguera; Mario Mencia; Margarita Salas;

  • 作者单位

    Centra de Biologia Molecular "Severo Ochoa", Consejo Superior de Investigaciones Cientfficas (CSIC)-Universidad Autdnoma de Madrid, Universidad Autonoma, Cantoblanco, 28049 Madrid, Spain;

    Centra de Biologia Molecular "Severo Ochoa", Consejo Superior de Investigaciones Cientfficas (CSIC)-Universidad Autdnoma de Madrid, Universidad Autonoma, Cantoblanco, 28049 Madrid, Spain,Tumor Suppression Group, Spanish National Cancer Centre (CNIO), 28029 Madrid, Spain;

    Centra de Biologia Molecular "Severo Ochoa", Consejo Superior de Investigaciones Cientfficas (CSIC)-Universidad Autdnoma de Madrid, Universidad Autonoma, Cantoblanco, 28049 Madrid, Spain;

    Centra de Biologia Molecular "Severo Ochoa", Consejo Superior de Investigaciones Cientfficas (CSIC)-Universidad Autdnoma de Madrid, Universidad Autonoma, Cantoblanco, 28049 Madrid, Spain;

    Centra de Biologia Molecular "Severo Ochoa", Consejo Superior de Investigaciones Cientfficas (CSIC)-Universidad Autdnoma de Madrid, Universidad Autonoma, Cantoblanco, 28049 Madrid, Spain;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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