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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Epistasis in tomato color mutations involves regulation of phytoene synthase 1 expression by cis-carotenoids
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Epistasis in tomato color mutations involves regulation of phytoene synthase 1 expression by cis-carotenoids

机译：番茄颜色突变中的上位性涉及顺式类胡萝卜素对六氢番茄红素合酶1表达的调节

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摘要

Tomato (Solarium lycopersicum) fruit accumulate the red carotenoid pigment lycopene. The recessive mutation yellow-flesh (locus r) in tomato eliminates fruit carotenoids by disrupting the activity of the fruit-specific phytoene synthase (PSY1), the first committed step in the carotenoid biosynthesis pathway. Fruits of the recessive mutation tangerine (t) appear orange due to accumulation of 7,9,7',9'-tetra-cis-lycopene (prolycopene) as a result of a mutation in the carotenoid cis-trans isomerase. It was established 60 y ago that tangerine is epistatic to yellow-flesh. This uncharacteristic epistasis interaction defies a paradigm in biochemical genetics arguing that mutations that disrupt enzymes acting early in a biosynthetic pathway are epistatic to other mutations that block downstream steps in the same pathway. To explain this conundrum, we have investigated the interaction between tangerine and yellow-flesh at the molecular level. Results presented here indicate that allele r~(2997) of yellow-flesh eliminates transcription of PSY1 in fruits. In a genetic background of tangerine, transcription of PSY1 is partially restored to a level sufficient for producing phytoene and downstream carotenoids. Our results revealed the molecular mechanism underlying the epistasis of t over r and suggest the involvement of cis-caroten-oid metabolites in a feedback regulation of PSY1 gene expression.
机译：番茄（Solarium lycopersicum）果实积聚红色类胡萝卜素色素番茄红素。番茄中的隐性突变黄肉（基因座r）通过破坏类胡萝卜素生物合成途径的第一个重要步骤-特定于水果的八氢番茄红素合酶（PSY1）的活性，消除了类胡萝卜素。隐性突变的橘子（t）的果实由于类胡萝卜素顺反异构酶的突变而积累了7,9,7'，9'-四顺式番茄红素（原番茄红素）而显示为橙色。大约60年前就已经确定，橘子对黄肉是上位性的。这种没有特征的上位相互作用对生物化学遗传学的范式提出了挑战，认为破坏破坏生物合成途径早期作用的酶的突变对于阻止同一途径下游步骤的其他突变是上位的。为了解释这个难题，我们在分子水平上研究了橘子和黄肉之间的相互作用。结果表明，黄肉等位基因r〜（2997）消除了果实中PSY1的转录。在橘子的遗传背景中，PSY1的转录部分恢复到足以产生八氢番茄红素和下游类胡萝卜素的水平。我们的研究结果揭示了t高于r上位性的分子机制，并暗示了顺式-类胡萝卜素代谢产物参与PSY1基因表达的反馈调节。

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    David E. Kachanovsky; Shdema Filler; Tal Isaacson; Joseph Hirschberg;

  • 作者单位

    Department of Genetics, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem 91904, Israel;

    Department of Genetics, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem 91904, Israel;

    Department of Genetics, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem 91904, Israel,Department of Fruit Tree Sciences, Agricultural Research Organization, Newe Ya'ar Research Center, Ramat Yishay 30095, Israel;

    Department of Genetics, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem 91904, Israel;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    fruit development; tomato breeding;

    机译：果实发育;番茄育种;

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