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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Controlling interactions in supported bilayers from weak electrostatic repulsion to high osmotic pressure
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Controlling interactions in supported bilayers from weak electrostatic repulsion to high osmotic pressure

机译：控制从弱静电排斥到高渗透压的支撑双层中的相互作用

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摘要

Understanding interactions between membranes requires measurements on well-controlled systems close to natural conditions, in which fluctuations play an important role. We have determined, by grazing incidence X-ray scattering, the interaction potential between two lipid bilayers, one adsorbed on a solid surface and the other floating close by. We find that interactions in this highly hydrated model system are two orders of magnitude softer than in previously reported work on multilayer stacks. This is attributed to the weak electrostatic repulsion due to the small fraction of ionized lipids in supported bilayers with a lower number of defects. Our data are consistent with the Poisson-Boltzmann theory, in the regime where repulsion is dominated by the entropy of counter ions. We also have unique access to very weak entropic repulsion potentials, which allowed us to discriminate between the various models proposed in the literature. We further demonstrate that the interaction potential between supported bilayers can be tuned at will by applying osmotic pressure, providing a way to manipulate these model membranes, thus considerably enlarging the range of biological or physical problems that can be addressed.
机译：了解膜之间的相互作用需要在接近自然条件的良好控制系统上进行测量，其中波动起着重要作用。通过掠射X射线散射，我们已经确定了两个脂质双层之间的相互作用势，其中一个脂质双层吸附在固体表面上，另一个在附近浮动。我们发现，在这种高度水合的模型系统中，交互作用比以前报道的多层堆栈中的交互作用要软两个数量级。这归因于弱的静电排斥力，这是由于负载的双层中离子脂质的比例很小，而缺陷数量较少。我们的数据与Poisson-Boltzmann理论是一致的，在这种情况下，推斥力由反离子的熵决定。我们还可以非常弱地利用熵排斥力，这使我们能够区分文献中提出的各种模型。我们进一步证明，可以通过施加渗透压随意调节支持的双层之间的相互作用潜力，从而提供一种操作这些模型膜的方法，从而大大扩大了可以解决的生物学或物理问题的范围。

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  • 作者

    Arnaud Hemmerle; Linda Malaquin; Thierry Charitat; Sigolene Lecuyer; Giovanna Fragneto; Jean Daillant;

  • 作者单位

    Universite de Strasbourg, Institut Charles Sadron, Unite Propre de Recherche 22, Centre National de la Recherche Scientifique (CNRS), 67034 Strasbourg Cedex 2, France,Laboratoire Interdisciplinaire sur I'Organisation Nanometrique et Supramoleculaire (LIONS), Service Interdisciplinaire sur les Systemes Moleculaires et les Materiaux (SIS2M), Institut Rayonnement Matiere de Saclay (IRAMIS), Commissariat a I'Energie Atomique (CEA), Unite Mixte de Recherche 3299 CEA/CNRS, F-91191 Gif-sur-Yvette Cedex, France;

    Universite de Strasbourg, Institut Charles Sadron, Unite Propre de Recherche 22, Centre National de la Recherche Scientifique (CNRS), 67034 Strasbourg Cedex 2, France,Laboratoire Interdisciplinaire sur I'Organisation Nanometrique et Supramoleculaire (LIONS), Service Interdisciplinaire sur les Systemes Moleculaires et les Materiaux (SIS2M), Institut Rayonnement Matiere de Saclay (IRAMIS), Commissariat a I'Energie Atomique (CEA), Unite Mixte de Recherche 3299 CEA/CNRS, F-91191 Gif-sur-Yvette Cedex, France;

    Universite de Strasbourg, Institut Charles Sadron, Unite Propre de Recherche 22, Centre National de la Recherche Scientifique (CNRS), 67034 Strasbourg Cedex 2, France;

    Universite de Strasbourg, Institut Charles Sadron, Unite Propre de Recherche 22, Centre National de la Recherche Scientifique (CNRS), 67034 Strasbourg Cedex 2, France,Laboratoire Interdisciplinaire de Physique, Unite Mixte de Recherche, Universite Joseph Fourier, and Centre National de la Recherche Scientifique, 38402 Saint Martin d'Heres, France;

    lnstitut Laue-Langevin, 38042 Grenoble Cedex, France;

    Laboratoire Interdisciplinaire sur I'Organisation Nanometrique et Supramoleculaire (LIONS), Service Interdisciplinaire sur les Systemes Moleculaires et les Materiaux (SIS2M), Institut Rayonnement Matiere de Saclay (IRAMIS), Commissariat a I'Energie Atomique (CEA), Unite Mixte de Recherche 3299 CEA/CNRS, F-91191 Gif-sur-Yvette Cedex, France,Synchrotron SOLEIL, L'Orme des Merisiers,Saint-Aubin, F-91192 Gif-sur-Yvette Cedex, France;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    interbilayer forces; statistical physics; electrostatic interaction;

    机译：双层间力;统计物理;静电相互作用;

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