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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Arabidopsis LATERAL ORGAN BOUNDARIES negatively regulates brassinosteroid accumulation to limit growth in organ boundaries
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Arabidopsis LATERAL ORGAN BOUNDARIES negatively regulates brassinosteroid accumulation to limit growth in organ boundaries

机译：拟南芥的横向器官边界负面调节油菜素类固醇的积累以限制器官边界的生长

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摘要

Leaves and flowers begin life as outgrowths from the edges of shoot apical meristems. Stem cell divisions in the meristem center replenish cells that are incorporated into organ primordia at the meristem periphery and leave the meristem. Organ boundaries, regions of limited growth that separate forming organs from the meristem, serve to isolate, these two domains and are critical for coordination of organogenesis and meristem maintenance. Boundary formation and maintenance are poorly understood processes, despite the identification of a number of boundary-specific transcription factors. Here we provide genetic and biochemical evidence that the Arabidopsis thaliana transcription factor LATERAL ORGAN BOUNDARIES (LOB) negatively regulates accumulation of the plant steroid hormone brassinosteroid (BR) in organ boundaries. We found that ectopic expression of LOB results in reduced BR responses. We identified BAS1, which encodes a BR-inactivating enzyme, as a direct target of LOB transcriptional activation. Loss-of-function lob mutants exhibit organ fusions, and this phenotype is suppressed by expression of BAS1 under the LOB promoter, indicating that BR hyperaccumulation contributes to the lob mutant phenotype. In addition, LOB expression is BR regulated; therefore, LOB and BR form a feedback loop to modulate local BR accumulation in organ boundaries to limit growth in the boundary domain.
机译：叶子和花朵开始生长，是芽顶分生组织边缘的产物。分生组织中心的干细胞分裂补充了在分生组织外围并入器官原基的细胞，并离开了分生组织。器官边界是将形成器官与分生组织分开的生长受限的区域，用于隔离这两个域，对于协调器官发生和分生组织的维持至关重要。尽管鉴定了许多边界特异性转录因子，但是边界形成和维持是人们很少了解的过程。在这里，我们提供了遗传和生化证据，证明拟南芥转录因子LATERAL ORGAN BOUNDARIES（LOB）对器官边界内植物类固醇激素油菜素类固醇（BR）的蓄积负调控。我们发现LOB的异位表达导致BR反应降低。我们确定了编码BR失活酶的BAS1作为LOB转录激活的直接目标。功能丧失的lob突变体表现出器官融合，并且该表型被LOB启动子下BAS1的表达抑制，表明BR的过度积累有助于lob突变体的表型。另外，LOB表达受BR调节；因此，LOB和BR形成一个反馈环，以调节器官边界中局部BR的积累，从而限制边界域的生长。

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    Elizabeth M. Bell; Wan-ching Lin; Aman Y. Husbands; Lifeng Yu; Venkateswari Jaganatha; Barbara Jablonska; Amanda Mangeon; Michael M. Neff; Thomas Girke; Patricia S. Springer;

  • 作者单位

    Department of Botany and Plant Sciences, Center for Plant Cell Biology, University of California, Riverside, CA 92521;

    Department of Botany and Plant Sciences, Center for Plant Cell Biology, University of California, Riverside, CA 92521;

    Department of Botany and Plant Sciences, Center for Plant Cell Biology, University of California, Riverside, CA 92521,Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724;

    Department of Botany and Plant Sciences, Center for Plant Cell Biology, University of California, Riverside, CA 92521;

    Department of Botany and Plant Sciences, Center for Plant Cell Biology, University of California, Riverside, CA 92521;

    Department of Botany and Plant Sciences, Center for Plant Cell Biology, University of California, Riverside, CA 92521;

    Department of Botany and Plant Sciences, Center for Plant Cell Biology, University of California, Riverside, CA 92521,Laboratorio de Genomica Funcional e Transducao de Sinai, Departa- mento de Genetica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, CP 68011, 21941-970, Brazil;

    Department of Crop and Soil Sciences, Washington State University, Pullman, WA 99164;

    Department of Botany and Plant Sciences, Center for Plant Cell Biology, University of California, Riverside, CA 92521;

    Department of Botany and Plant Sciences, Center for Plant Cell Biology, University of California, Riverside, CA 92521;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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