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Birth and death of genes linked to chromosomal inversion

机译:与染色体倒置有关的基因的出生和死亡

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摘要

The birth and death of genes is central to adaptive evolution, yet the underlying genome dynamics remain elusive. The availability of closely related complete genome sequences helps to follow changes in gene contents and clarify their relationship to overall genome organization. Helicobacter pylori, bacteria in our stomach, are known for their extreme genome plasticity through mutation and recombination and will make a good target for such an analysis. In comparing their complete genome sequences, we found that gain and loss of genes (loci) for outer membrane proteins, which mediate host interaction, occurred at breakpoints of chromosomal inversions. Sequence comparison there revealed a unique mechanism of DNA duplication: DNA duplication associated with inversion. In this process, a DNA Segment at one chromosomal locus is copied and inserted, in an inverted orientation, into a distant locus on the same chromosome, while the entire region between these two loci is also inverted. Recognition of this and three more inversion modes, which occur through reciprocal recombination between long or short sequence similarity or adjacent to a mobile element, allowed reconstruction of synteny evolution through inversion events in this species. These results will guide the interpretation of extensive DNA sequencing results for understanding long- and short-term genome evolution in various organisms and in cancer cells.
机译:基因的诞生和死亡对于适应性进化至关重要,但是潜在的基因组动力学仍然难以捉摸。紧密相关的完整基因组序列的可用性有助于跟踪基因含量的变化,并阐明它们与整体基因组组织的关系。幽门螺杆菌是我们胃中的细菌,因突变和重组而具有极高的基因组可塑性,因此将成为此类分析的良好靶标。通过比较它们的完整基因组序列,我们发现介导宿主相互作用的外膜蛋白基因(基因)的获得和丢失发生在染色体倒置的断点处。那里的序列比较揭示了DNA复制的独特机制:与倒置相关的DNA复制。在此过程中,将一个染色体基因座处的DNA片段复制并以相反的方向插入到同一条染色体上的远处基因座中,而这两个基因座之间的整个区域也都被颠倒了。通过长或短序列相似性之间的相互重组或与移动元件相邻发生的这种和另外三种反转模式的识别,使得可以通过该物种中的反转事件重建同义进化。这些结果将指导广泛的DNA测序结果的解释,以了解各种生物体和癌细胞中长期和短期的基因组进化。

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  • 作者单位

    Department of Medical Genome Sciences, Graduate School of Frontier Sciences, University of Tokyo, Minato-ku, Tokyo 108-8639, Japan,Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo 108-8639, Japan;

    Department of Medical Genome Sciences, Graduate School of Frontier Sciences, University of Tokyo, Minato-ku, Tokyo 108-8639, Japan,Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo 108-8639, Japan,Laboratory of Genome Informatics, National Institute for Basic Biology, National Institutes of Natural Sciences, Okazaki, Aichi 444-8585, Japan;

    Graduate School of Medicine, Kurume University, Kurume, Fukuoka 830-0011, Japan,Fujitsu Kyushu Systems Ltd., Fukuoka, Fukuoka 814-8589, Japan;

    Department of Medical Genome Sciences, Graduate School of Frontier Sciences, University of Tokyo, Minato-ku, Tokyo 108-8639, Japan,Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo 108-8639, Japan;

    Department of Medical Genome Sciences, Graduate School of Frontier Sciences, University of Tokyo, Minato-ku, Tokyo 108-8639, Japan,Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo 108-8639, Japan;

    Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo 108-8639, Japan,Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Minato-ku, Tokyo 108-8639, Japan;

    Department of Computational Biology, Graduate School of Frontier Sciences, University of Tokyo, Kashiwa, Chiba 277-8561, Japan;

    Department of Gastroenterology, Graduate School of Medicine, Kobe University, Chuou-ku, Kobe, Hyogo 650-0017, Japan;

    Department of Gastroenterology, Graduate School of Medicine, Kobe University, Chuou-ku, Kobe, Hyogo 650-0017, Japan;

    Department of Computational Biology, Graduate School of Frontier Sciences, University of Tokyo, Kashiwa, Chiba 277-8561, Japan;

    Laboratory of Genome Informatics, National Institute for Basic Biology, National Institutes of Natural Sciences, Okazaki, Aichi 444-8585, Japan;

    Department of Medical Genome Sciences, Graduate School of Frontier Sciences, University of Tokyo, Minato-ku, Tokyo 108-8639, Japan,Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo 108-8639, Japan,Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Minato-ku, Tokyo 108-8639, Japan;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    genome comparison; gene duplication; genome rearrangement; structural variation; replicative inversion;

    机译:基因组比较;基因复制;基因组重排;结构变异;复制反转;

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