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Membrane lipidome of an epithelial cell line

机译:上皮细胞系的膜脂质组

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摘要

Tissue differentiation is an important process that involves major cellular membrane remodeling. We used Madin-Darby canine kidney cells as a model for epithelium formation and investigated the remodeling of the total cell membrane lipidome during the transition from a nonpolarized morphology to an epithelial morphology and vice versa. To achieve this, we developed a shotgun-based lipidomics workflow that enabled the absolute quantification of mammalian membrane lipidomes with minimal sample processing from low sample amounts. Epithelial morphogenesis was accompanied by a major shift from sphingomyelin to glycosphingolipid, together with an increase in plasmalogen, phosphatidylethanol-amine, and cholesterol content, whereas the opposite changes took place during an epithelial-to-mesenchymal transition. Moreover, during polarization, the sphingolipids became longer, more saturated, and more hydroxylated as required to generate an apical membrane domain that serves as a protective barrier for the epithelial sheet.
机译:组织分化是涉及主要细胞膜重塑的重要过程。我们使用Madin-Darby犬肾细胞作为上皮形成的模型,并研究了从非极化形态到上皮形态转变(反之亦然)期间总细胞膜脂质组的重塑。为了实现这一目标,我们开发了一种基于散弹枪的脂质组学工作流程,该流程可对哺乳动物膜脂质组进行绝对定量,并从少量样品中进行最少的样品处理。上皮形态发生伴随着从鞘磷脂到糖鞘脂的重大转变,同时缩醛磷脂,磷脂酰乙醇胺和胆固醇含量增加,而相反的变化发生在上皮向间充质转变期间。而且,在极化过程中,鞘脂视需要变得更长,更饱和并且被更多地羟基化,以产生用作上皮片的保护屏障的顶膜结构域。

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