...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Holliday junction-containing DNA structures persist in cells lacking Sgs1 or Top3 following exposure to DNA damage
【24h】

Holliday junction-containing DNA structures persist in cells lacking Sgs1 or Top3 following exposure to DNA damage

机译：在暴露于DNA损伤后缺乏Sgs1或Top3的细胞中仍存在含有霍利迪接头的DNA结构

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The Sgs1-Rmi1-Top3 "dissolvasome" is required for the maintenance of genome stability and has been implicated in the processing of various types of DNA structures arising during DNA replication. Previous investigations have revealed that unprocessed (X-shaped) homologous recombination repair (HRR) intermediates persist when S-phase is perturbed by using methyl methanesulfonate (MMS) in Saccharomyces cerevisiae cells with impaired Sgs1 or Top3. However, the precise nature of these persistent DNA structures remains poorly characterized. Here, we report that ectopic expression of either of two heterologous and structurally unrelated Holliday junction (HJ) resolvases, Escherichia coli RusA or human GEN1~(1-527), promotes the removal of these X-structures in vivo. Moreover, other types of DNA replication intermediates, including stalled replication forks and non-HRR-dependent X-structures, are refractory to RusA or GEN1~(1-527), demonstrating specificity of these HJ resolvases for MMS-induced X-structures in vivo. These data suggest that the X-structures persisting in cells with impaired Sgs1 or Top3 contain HJs. Furthermore, we demonstrate that Sgs1 directly promotes X-structure removal, because the persistent structures arising in Sgs1-deficient strains are eliminated when Sgs1 is reactivated in vivo. We propose that HJ resolvases and Sgs1-Top3-Rmi1 comprise two independent processes to deal with HJ-containing DNA intermediates arising during HRR in S-phase.
机译：Sgs1-Rmi1-Top3“溶解体”是维持基因组稳定性所必需的，并且与DNA复制过程中产生的各种类型的DNA结构的加工有关。先前的研究表明，当在Sgchar1或Top3受损的酿酒酵母细胞中使用甲磺酸甲酯（MMS）干扰S期时，未加工的（X形）同源重组修复（HRR）中间体仍然存在。但是，这些持久性DNA结构的精确性质仍然不好表征。在这里，我们报告两个异源和结构上不相关的霍利迪结（HJ）解决方案，大肠杆菌RusA或人类GEN1〜（1-527）异位表达促进体内这些X结构的删除。此外，其他类型的DNA复制中间体，包括停滞的复制叉和非HRR依赖性X结构，对RusA或GEN1〜（1-527）均具有耐药性，表明这些HJ拆分物对MMS诱导的X结构具有特异性。体内。这些数据表明，Sgs1或Top3受损的细胞中持续存在的X结构含有HJ。此外，我们证明Sgs1直接促进X结构的去除，因为当在体内重新激活Sgs1时，消除了Sgs1缺陷菌株中产生的持久结构。我们建议HJ resolvases和Sgs1-Top3-Rmi1包括两个独立的过程，以处理在S期HRR过程中产生的含HJ的DNA中间体。

著录项

  • 来源
  • 作者

    Hocine W. Mankouri; Thomas M. Ashton; Ian D. Hickson;

  • 作者单位

    Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford 0X3 9DS, United Kingdom,Nordea Center for Healthy Aging, Department of Cellular and Molecular Medicine, University of Copenhagen, 2200 Copenhagen N, Denmark;

    Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford 0X3 9DS, United Kingdom;

    Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford 0X3 9DS, United Kingdom,Nordea Center for Healthy Aging, Department of Cellular and Molecular Medicine, University of Copenhagen, 2200 Copenhagen N, Denmark;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    DNA repair; RecQ; helicase; topoisomerase;

    机译：DNA修复;RecQ;解旋酶;拓扑异构酶;

相似文献

  • 外文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号