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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Experimental illumination of a fitness landscape
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Experimental illumination of a fitness landscape

机译：健身景观的实验照明

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The genes of all organisms have been shaped by selective pressures. The relationship between gene sequence and fitness has tremendous implications for understanding both evolutionary processes and functional constraints on the encoded proteins. Here, we have exploited deep sequencing technology to experimentally determine the fitness of all possible individual point mutants under controlled conditions for a nine-amino acid region of Hsp90. Over the past five decades, limited glimpses into the relationship between gene sequence and function have sparked a long debate regarding the distribution, relative proportion, and evolutionary significance of deleterious, neutral, and advantageous mutations. Our systematic experimental measurement of fitness effects of Hsp90 mutants in yeast, evaluated in the light of existing population genetic theory, are remarkably consistent with a nearly neutral model of molecular evolution.
机译：所有生物的基因都受到选择压力的影响。基因序列与适应性之间的关系对于理解进化过程和编码蛋白的功能限制都具有重大意义。在这里，我们已经利用深度测序技术来实验确定Hsp90的9个氨基酸区域在受控条件下所有可能的单个点突变体的适合度。在过去的五十年中，对基因序列与功能之间关系的有限了解引起了关于有害，中性和有利突变的分布，相对比例和进化重要性的长期争论。根据现有的种群遗传理论，我们对Hsp90突变体在酵母中的适应性作用进行了系统的实验测量，与近乎中性的分子进化模型非常一致。

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