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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Stage-specific proteomic expression patterns of the human filarial parasite Brugia malayi and its endosymbiont Wolbachia
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Stage-specific proteomic expression patterns of the human filarial parasite Brugia malayi and its endosymbiont Wolbachia

机译：人类丝虫寄生虫马来亚布鲁氏菌及其内共生沃尔巴克氏菌的阶段特异性蛋白质组表达模式

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摘要

Global proteomic analyses of pathogens have thus far been limited to unicellular organisms (e.g., protozoa and bacteria). Proteomic analyses of most eukaryotic pathogens (e.g., helminths) have been restricted to specific organs, specific stages, or secretomes. We report here a large-scale proteomic characterization of almost all the major mammalian stages of Brugia malayi. a causative agent of lymphatic filariasis, resulting in the identification of more than 62% of the products predicted from the Bm draft genome. The analysis also yielded much of the proteome of Wolbachia, the obligate endosymbiont of Bm that also expressed proteins in a stage-specific manner. Of the 11,610 predicted Bm gene products, 7,103 were definitively identified frpm adult male, adult female, blood-borne and uterine microfilariae, and infective L3 larvae. Among the 4,956 gene products (42.5%) inferred from the genome as "hypothetical," the present study was able to confirm 2,336 (47.1%) as bona fide proteins. Analysis of protein families and domains coupled with stage-specific expression highlight the important pathways that benefit the parasite during its development in the host. Gene set enrichment analysis identified extracellular matrix proteins and those with immunologic effects as enriched in the microfilarial and L3 stages. Parasite sex- and stage-specific protein expression identified those pathways related to parasite differentiation and demonstrates stage-specific expression by the Bm endosymbiont Wolbachia as well.
机译：迄今为止，对病原体的整体蛋白质组学分析仅限于单细胞生物（例如原生动物和细菌）。大多数真核病原体（例如蠕虫）的蛋白质组分析仅限于特定器官，特定阶段或分泌组。我们在这里报告了布鲁贾亚马来虫的几乎所有主要哺乳动物阶段的大规模蛋白质组学表征。是淋巴丝虫病的病原体，导致从Bm草图基因组预测的产物中鉴定出62％以上。该分析还产生了Wolbachia的许多蛋白质组，Wolbachia是Bm的专性内共生体，它也以阶段特异性方式表达蛋白质。在11,610个预测的Bm基因产物中，有7,103个被明确确定为frpm成年男性，成年女性，血源性和子宫微丝虫，以及感染性L3幼虫。在从基因组中推断为“假设的”的4956种基因产物中（42.5％），本研究能够确认2336种（47.1％）为真正的蛋白质。对蛋白质家族和结构域的分析以及阶段特异性表达突出了在宿主体内寄生虫发育期间有益于寄生虫的重要途径。基因集富集分析确定了细胞外基质蛋白以及具有免疫作用的蛋白，这些蛋白在微丝和L3期富集。寄生虫的性别和阶段特异性蛋白表达确定了与寄生虫分化相关的那些途径，并通过Bm内共生菌Wolbachia证明了阶段特异性表达。

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    Sasisekhar Bennuru; Zhaojing Meng; Jose M. C. Ribeiro; Roshanak Tolouei Semnani; Elodie Ghedin; King Chan; David A. Lucas; Timothy D. Veenstra; Thomas B. Nutman;

  • 作者单位

    Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892;

    Laboratory of Proteomics and Analytical Technologies, Advanced Technology Program, SAIC-Frederick, Frederick, MD 21702;

    Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892;

    Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892;

    Center for Vaccine Research, Department of Computational and Systems Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261;

    Laboratory of Proteomics and Analytical Technologies, Advanced Technology Program, SAIC-Frederick, Frederick, MD 21702;

    Laboratory of Proteomics and Analytical Technologies, Advanced Technology Program, SAIC-Frederick, Frederick, MD 21702;

    Laboratory of Proteomics and Analytical Technologies, Advanced Technology Program, SAIC-Frederick, Frederick, MD 21702;

    Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    filaria; nematode;

    机译：丝aria线虫;

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