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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Dual amyloid domains promote differential functioning of the chaplin proteins during Streptomyces aerial morphogenesis
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Dual amyloid domains promote differential functioning of the chaplin proteins during Streptomyces aerial morphogenesis

机译：淀粉样蛋白双结构域在链霉菌空中形态发生过程中促进卓别林蛋白的差异功能

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摘要

The chaplin proteins are functional amyloids found in the filamen tous Streptomyces bacteria. These secreted proteins are required for the aerial development of Streptomyces coelicolor, and con tribute to an intricate rodlet ultrastructure that decorates the surfaces of aerial hyphae and spores. 5. coelicolor encodes eight chaplin proteins. Previous studies have revealed that only three of these proteins (ChpC, ChpE, and ChpH) are necessary for promot ing aerial development, and of these three, ChpH is the primary developmental determinant. Here, we show that the model chaplin, ChpH, contains two amyloidogenic domains: one in the N terminus and one in the C terminus of the mature protein. These domains have different polymerization properties as determined using fluorescence spectroscopy, secondary structure analyses, and electron microscopy. We coupled these in vitro assays with in vivo genetic studies to probe the connection between ChpH amyloidogenesis and its biological function. Using mutational analyses, we demonstrated that both N- and C-terminal amyloid domains of ChpH were required for promoting aerial hypha forma tion, while the N-terminal domain was dispensable for assembly of the rodlet ultrastructure. These results suggest that there is a functional differentiation of the dual amyloid domains in the chaplin proteins.
机译：卓别林蛋白是在丝状链霉菌细菌中发现的功能淀粉样蛋白。这些分泌的蛋白质是天蓝色链霉菌的空中发育所必需的，并且有助于装饰气生菌丝和孢子表面的复杂的小杆超微结构。 5. coelicolor编码八种卓别林蛋白。先前的研究表明，这些蛋白质中只有三种（ChpC，ChpE和ChpH）对于促进空中发育是必需的，而在这三种蛋白质中，ChpH是主要的发育决定因素。在这里，我们显示模型卓别林ChpH包含两个淀粉样蛋白生成域：一个在成熟蛋白的N末端，一个在C末端。这些域具有不同的聚合特性，如使用荧光光谱，二级结构分析和电子显微镜确定的。我们将这些体外测定与体内遗传研究相结合，以探索ChpH淀粉样蛋白生成与其生物学功能之间的联系。使用突变分析，我们证明了ChpH的N和C末端淀粉样蛋白域都是促进气生菌丝形成所必需的，而N末端域对于组装小杆超微结构是必不可少的。这些结果表明，卓别林蛋白中的双淀粉样蛋白结构域存在功能分化。

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    David S. Capstick; Ahmad Jomaa; Chistopher Hanke; Joaquin Ortega; Marie A. Elliot;

  • 作者单位

    Department of Biology and M.G. DeGroote Institute for Infectious Disease Research, McMaster University, 1280 Main Street West, Hamilton, ON, Canada L8S 4K1;

    Department of Biochemistry and Biomedical Sciences and M.G. DeGroote Institute for Infectious Disease Research, McMaster University,1200 Main Street West, Hamilton, ON, Canada L8N 3Z5;

    Department of Biology and M.G. DeGroote Institute for Infectious Disease Research, McMaster University, 1280 Main Street West, Hamilton, ON, Canada L8S 4K1;

    Department of Biochemistry and Biomedical Sciences and M.G. DeGroote Institute for Infectious Disease Research, McMaster University,1200 Main Street West, Hamilton, ON, Canada L8N 3Z5;

    Department of Biology and M.G. DeGroote Institute for Infectious Disease Research, McMaster University, 1280 Main Street West, Hamilton, ON, Canada L8S 4K1;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    streptomyces development; sporulation; surface ultrastructure;

    机译：链霉菌发育;孢子形成;表面超微结构;

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