...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Interaction of complexes I, III, and IV within the bovine respirasome by single particle cryoelectron tomography
【24h】

Interaction of complexes I, III, and IV within the bovine respirasome by single particle cryoelectron tomography

机译：单粒子低温电子层析成像技术在牛呼吸体中复合物I，III和IV的相互作用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The respirasome is a multisubunit supercomplex of the respiratory chain in mitochondria. Here we report the 3D reconstruction of the bovine heart respirasome, composed of dimeric complex III and single copies of complex I and IV, at about 2.2-nm resolution, determined by cryoelectron tomography and subvolume averaging. Fitting of X-ray structures of single complexes I, lll2, and IV with high fidelity allows interpretation of the model at the level of secondary structures and shows how the individual complexes interact within the respirasome. Surprisingly, the distance between cytochrome c binding sites of complexes lll2 and IV is about 10 nm. Modeling indicates a loose interaction between the three complexes and provides evidence that lipids are gluing them at the interfaces.
机译：呼吸小体是线粒体中呼吸链的多亚基超复合体。在这里，我们报告了由冷冻电子体层摄影术和子体积平均法确定的约2.2 nm分辨率的由二聚体复合物III和复合物I和IV的单拷贝组成的牛心脏呼吸体的3D重建。以高保真度拟合单个复合物I，III2和IV的X射线结构，可以在二级结构的水平上解释模型，并显示单个复合物如何在呼吸体中相互作用。令人惊讶地，复合物1112和IV的细胞色素c结合位点之间的距离为约10nm。建模表明这三种复合物之间存在松散的相互作用，并提供证据表明脂质在界面处将其胶合。

著录项

  • 来源
  • 作者

    Natalya V. Dudkina; Mikhail Kudryashev; Henning Stahlberg; Egbert J. Boekema;

  • 作者单位

    Electron Microscopy Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 7, 9747AG, Groningen,The Netherlands;

    Center for Cellular Imaging and Nano Analytics, University of Basel, Mattenstrasse 26, CH-4058 Basel, Switzerland;

    Center for Cellular Imaging and Nano Analytics, University of Basel, Mattenstrasse 26, CH-4058 Basel, Switzerland;

    Electron Microscopy Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 7, 9747AG, Groningen,The Netherlands;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    electron microscopy; oxidative phosphorylation;

    机译：电子显微镜;氧化磷酸化;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号