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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Free radical functionalization of surfaces to prevent adverse responses to biomedical devices
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Free radical functionalization of surfaces to prevent adverse responses to biomedical devices

机译:表面的自由基功能化,以防止对生物医学设备的不良反应

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Immobilizing a protein, that is fully compatible with the patient, on the surface of a biomedical device should make it possible to avoid adverse responses such as inflammation, rejection, or excessive fibrosis. A surface that strongly binds and does not denature the compatible protein is required. Hydrophilic surfaces do not induce denaturation of immobilized protein but exhibit a low binding affinity for protein. Here, .we describe an energetic ion-assisted plasma process that can make any surface hydrophilic and at the same time enable it to covalently immobilize functional biological molecules. We show that the modification creates free radicals that migrate to the surface from a reservoir beneath. When they reach the surface, the radicals form covalent bonds with biomolecules. The kinetics and number densities of protein molecules in solution and free radicals in the reservoir control the time required to form a full protein monolayer that is covalently bound. The shelf life of the covalent binding capability is governed by the initial density of free radicals and the depth of the reservoir. We show that the high reactivity of the radicals renders the binding universal across all biological macromolecules. Because the free radical reservoir can be created on any solid material, this approach can be used in medical applications ranging from cardiovascular stents to heart-lung machines.
机译:将与患者完全相容的蛋白质固定在生物医学设备的表面上,应该可以避免不良反应,例如炎症,排斥反应或过度纤维化。需要牢固结合但不会使兼容蛋白质变性的表面。亲水性表面不会诱导固定化蛋白质变性,但对蛋白质的结合亲和力较低。在这里,我们描述了一种高能离子辅助等离子体工艺,该工艺可以使任何表面都具有亲水性,同时使其能够共价固定功能性生物分子。我们表明,这种修饰会产生自由基,这些自由基会从下面的储层迁移到表面。当它们到达表面时,自由基与生物分子形成共价键。溶液中蛋白质分子的动力学和数量密度以及储层中的自由基控制形成共价结合的完整蛋白质单层所需的时间。共价结合能力的保质期由自由基的初始密度和储库的深度决定。我们表明,自由基的高反应性使得结合在所有生物大分子上具有普遍性。由于自由基储存库可以在任何固体材料上生成,因此该方法可用于医疗应用,从心血管支架到心肺机。

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