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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Quantitative ratiometric discrimination between noncancerous and cancerous prostate cells based on neuropilin-1 overexpression
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Quantitative ratiometric discrimination between noncancerous and cancerous prostate cells based on neuropilin-1 overexpression

机译：基于Neuropilin-1过表达的非癌性和癌性前列腺细胞之间的定量比例鉴别

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摘要

A multiplexed, ratiometric method is described that can confi dently distinguish between cancerous and noncancerous epithelial prostate cells in vitro. The technique is based on bright surface enhanced resonance Raman scattering (SERRS) biotags (SBTs) infused with unique Raman reporter molecules, and carrying cell specific peptides. Two sets of SBTs were used. One targets the neuropilin-1 (NRP-1) receptors of cancer cells through the RPARPAR peptide. The other functions as a positive control (PC) and binds to both noncancerous and cancer cells through the HIV-derived TAT peptide. Point-by-point 2D Raman maps of the spatial distribution of the two tags were constructed with subcellular resolution from cells simultaneously incubated with the two sets of SBTs. Aver aging the SERRS signal over a given cell yielded an NRP/PC ratio from which a robust quantitative measure of the overexpression of the NRP-1 by the cancer cell line was extracted. The use of a local, on-cell reference produces quantitative, statistically robust measures of overexpression independent of such sources of uncer tainty as variations in the location of the focal plane, the local cell concentration, and turbidity.
机译：描述了一种可以在体外可靠地区分癌性和非癌性上皮前列腺细胞的多重比例法。该技术基于注入了独特的拉曼报告分子并携带细胞特异性肽的亮表面增强共振拉曼散射（SERRS）生物标签（SBT）。使用了两组SBT。一种通过RPARPAR肽靶向癌细胞的Neuropilin-1（NRP-1）受体。另一个用作阳性对照（PC），并通过HIV衍生的TAT肽与非癌细胞和癌细胞结合。从同时与两组SBT一起孵育的细胞中获得亚细胞分辨率，构建两个标签的空间分布的逐点2D拉曼图。在给定细胞上对SERRS信号进行平均老化会产生NRP / PC比，从中提取出癌细胞系NRP-1过表达的可靠定量方法。使用局部细胞内参考可产生定量的，统计学上可靠的过表达量度，而与诸如焦平面位置，局部细胞浓度和浊度变化等不确定性来源无关。

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