Lower specific infectivity of protease-resistant priori protein generated in cell-free reactions

Mikael Klingeborn; Brent Race; Kimberly D. Meade-White; Bruce Chesebro

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Lower specific infectivity of protease-resistant priori protein generated in cell-free reactions

机译：在无细胞反应中产生的蛋白酶抗性先验蛋白的比感染性较低

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Prions are unconventional agents that infect animals and humans and cause a family of degenerative brain diseases known as transmissible spongi-form encephalopathy or prion diseases. It is difficult to inactivate prions so that they cannot infect hosts, and the biochemical nature of prions has not been precisely determined. In previous experiments using a protein mis-folding cyclic amplification (PMCA) method, where mis-folding of normal prion protein was initiated in cell-free test tube reactions by addition of a small amount of misfolded disease-associated protease-resistant prion protein (PrPres), both infectivity and PrPres were generated.

机译：ions病毒是一种非常规药物，会感染动物和人类，并导致一类退化性脑病，称为传染性海绵状脑病或病毒病。病毒很难失活，以致它们无法感染宿主，and病毒的生化性质尚未精确确定。在先前使用蛋白质错误折叠循环扩增（PMCA）方法进行的实验中，正常的病毒蛋白质的错误折叠是在无细胞试管反应中通过添加少量错误折叠的与疾病相关的蛋白酶抗性病毒蛋白质而引发的（ PrPres），同时产生感染性和PrPres。

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来源
《Proceedings of the National Academy of Sciences of the United States of America》 |2011年第48期|p.19109-19110|共2页
作者
Mikael Klingeborn; Brent Race; Kimberly D. Meade-White; Bruce Chesebro;
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作者单位

Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, MT 59840;

Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, MT 59840;

Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, MT 59840;

Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, MT 59840;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
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专利

1. Strain-specific effects of reducing agents on the cell-free conversion of recombinant prion protein into a protease-resistant form [J] . Imamura Morikazu, Kato Nobuko, Okada Hiroyuki, Microbiology and Immunology . 2011,第9期

机译：还原剂对重组病毒蛋白无细胞转化为蛋白酶抗性形式的菌株特异性作用
2. Species specificity in the cell-free conversion of prion protein to protease-resistant forms: a model for the scrapie species barrier. [J] . Kocisko DA, Priola SA, Raymond GJ, Proceedings of the National Academy of Sciences of the United States of America . 1995,第9期

机译：病毒蛋白无细胞转化为蛋白酶抗性形式的物种特异性：痒病物种屏障的模型。
3. The effect of Fenton reaction on protease-resistant prion protein (PrPSc) degradation and scrapie infectivity. [J] . Park SJ, Kim NH, Jeong BH, Brain research . 2008,第0期

机译：芬顿反应对蛋白酶抗朊病毒蛋白（PRPSC）降解和瘙痒病感染的影响。
4. Development of a Human Cell-Free Expression System to Generate Stable-Isotope-Labeled Protein Standards for Quantitative Mass Spectrometry [C] . Ryan D. Bomgarden, Derek Baerenwald, Eric Hommema, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2012

机译：一种无细胞表达系统的开发，以产生用于定量质谱法的稳定同位素标记的蛋白质标准
5. Analysis and control of central metabolic pathways during cell-free protein synthesis reactions. [D] . Calhoun, Kara Anne. 2006

机译：无细胞蛋白质合成反应过程中中央代谢途径的分析和控制。
6. PNAS Plus: Lower specific infectivity of protease-resistant prion protein generated in cell-free reactions [O] . Mikael Klingeborn, Brent Race, Kimberly D. Meade-White, 2011

机译：PNAS Plus：在无细胞反应中产生的蛋白酶抗性pr病毒蛋白的较低比感染性
7. Lower specific infectivity of protease-resistant prion protein generated in cell-free reactions [O] . Klingeborn, Mikael, Race, Brent, Meade-White, Kimberly D., 2011

机译：在无细胞反应中产生的蛋白酶抗性pr病毒蛋白的比感染性较低

Lower specific infectivity of protease-resistant priori protein generated in cell-free reactions

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