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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Mature clustered, regularly interspaced, short palindromic repeats RNA (crRNA) length is measured by a ruler mechanism anchored at the precursor processing site
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Mature clustered, regularly interspaced, short palindromic repeats RNA (crRNA) length is measured by a ruler mechanism anchored at the precursor processing site

机译：通过锚定在前体加工位点的标尺机制测量成熟的簇状，规则间隔的，短回文重复序列RNA（crRNA）的长度

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摘要

Precise RNA processing is fundamental to all small RNA-mediated interference pathways. In prokaryotes, clustered, regularly interspaced, short palindromic repeats (CRISPR) loci encode small CRISPR RNAs (crRNAs) that protect against invasive genetic elements by antisense targeting. CRISPR loci are transcribed as a long precursor that is cleaved within repeat sequences by CRISPR-associated (Cas) proteins. In many organisms, this primary processing generates crRNA intermediates that are subject to additional nucleolytic trimming to render mature crRNAs of specific lengths. The molecular mechanisms underlying this maturation event remain poorly understood. Here, we defined the genetic requirements for crRNA primary processing and maturation in Staphylococcus epidermidis. We show that changes in the position of the primary processing site result in extended or diminished maturation to generate mature crRNAs of constant length. These results indicate that crRNA maturation occurs by a ruler mechanism anchored at the primary processing site. We also show that maturation is mediated by specific cas genes distinct from those genes involved in primary processing, showing that this event is directed by CRISPR/Cas loci.
机译：精确的RNA处理对于所有小RNA介导的干扰途径都是至关重要的。在原核生物中，成簇的，规则间隔的短回文重复序列（CRISPR）位点编码小的CRISPR RNA（crRNA），可通过反义靶向来防御侵袭性遗传元素。 CRISPR基因座被转录为一个长的前体，可被CRISPR相关（Cas）蛋白在重复序列内切割。在许多生物中，这种主要加工过程产生了crRNA中间体，该中间体需要进行额外的溶核修饰以产生特定长度的成熟crRNA。这种成熟事件背后的分子机制仍然知之甚少。在这里，我们定义了表皮葡萄球菌中crRNA初级加工和成熟的遗传要求。我们表明，在主要加工位点的位置变化导致成熟的延长或减少，以生成恒定长度的成熟crRNA。这些结果表明，crRNA成熟是通过锚定在主要加工位点的标尺机制发生的。我们还显示，成熟是由特定的cas基因介导的，该基因不同于参与初级加工的那些基因，表明该事件是由CRISPR / Cas基因座指导的。

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