...
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Plant flotillins are required for infection by nitrogen-fixing bacteria
【24h】

Plant flotillins are required for infection by nitrogen-fixing bacteria

机译:固氮细菌感染需要植物花粉蛋白

获取原文
获取原文并翻译 | 示例
           

摘要

To establish compatible rhizobial-legume symbioses, plant roots support bacterial infection via host-derived infection threads (ITs). Here, we report the requirement of plant f lotillin-like genes (FLOTs) in Sinorhizobium meliloti infection of its host legume Medicago trun-catula. Flotillins in other organisms have roles in viral pathogenesis, endocytosis, and membrane shaping. We identified seven FLOT genes in the M. truncatula genome and show that two, FLOT2 and FLOT4, are strongly up-regulated during early symbiotic events. This up-regulation depends on bacterial Nod Factor and the plant's ability to perceive Nod Factor. Microscopy data suggest that M. truncatula FLOT2 and FLOT4 localize to membrane microdomains. Upon rhizo-bial inoculation, FLOT4 uniquely becomes localized to the tips of elongating root hairs. Silencing FLOT2 and FLOT4 gene expression reveals a nonredundant requirement for both genes in IT initiation and nodule formation. FLOT4 is uniquely required for IT elongation, and FLOT4 localizes to IT membranes. This work reveals a critical role for plant flotillins in symbiotic bacterial infection.
机译:为了建立相容的根瘤菌-豆科共生菌,植物根通过宿主衍生的感染线(IT)支持细菌感染。在这里,我们报告在其寄主豆科植物紫花苜蓿的苜蓿中华根瘤菌感染中需要植物f菌素样基因(FLOTs)。其他生物体中的絮凝素在病毒的发病机制,内吞作用和膜形成中起作用。我们在M. truncatula基因组中鉴定了七个FLOT基因,并显示了两个FLOT2和FLOT4在早期共生事件中被强烈上调。这种上调取决于细菌的Nod因子和植物感知Nod因子的能力。显微镜数据表明,截形衣原体FLOT2和FLOT4定位于膜微区。根瘤菌接种后,FLOT4独特地定位于伸长的根毛尖端。沉默FLOT2和FLOT4基因表达揭示了IT启动和结节形成过程中两个基因的非冗余需求。 FLOT4是IT延长所特有的,而FLOT4则局限于IT膜。这项工作揭示了植物絮凝素在共生细菌感染中的关键作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号