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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Blocking of Plasmodium transmission by cooperative action of Cecropin A and Defensin A in transgenic Aedes aegypti mosquitoes
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Blocking of Plasmodium transmission by cooperative action of Cecropin A and Defensin A in transgenic Aedes aegypti mosquitoes

机译：通过天蚕素A和防御素A在转基因埃及伊蚊中的协同作用来阻断疟原虫的传播

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摘要

To overcome burden of mosquito-borne diseases, multiple control strategies are needed. Population replacement with genetically modified mosquitoes carrying antipathogen effector genes is one of the possible approaches for controlling disease transmission. However, transgenic mosquitoes with antipathogen pheno-types based on overexpression of a single type effector molecule are not efficient in interrupting pathogen transmission. Here, we show that co-overexpression of two antimicrobial peptides (AMP), Cecropin A, and Defensin A, in transgenic Aedes aegypti mosquitoes results in the cooperative antibacterial and antiP/as-modium action of these AMPs. The transgenic hybrid mosquitoes that overexpressed both Cecropin A and Defensin A under the control of the vitellogenin promoter exhibited an elevated resistance to Pseudomonas aeruginosa infection, indicating that these AMPs acted cooperatively against this pathogenic bacterium. In these mosquitoes infected with P. gallinaceum, the number of oocysts was dramatically reduced in midguts, and no sporozoites were found in their salivary glands when the mosquitoes were fed twice to reactivate transgenic AMP production. Infection experiments using the transgenic hybrid mosquitoes, followed by sequential feeding on naive chicken, and then naive wild-type mosquitoes showed that the Plasmodium transmission was completely blocked. This study suggests an approach in generating transgenic mosquitoes with antiPlasmodium refractory phenotype, which is coexpression of two or more effector molecules with cooperative action on the parasite.
机译：为了克服蚊媒疾病的负担，需要多种控制策略。用携带抗病原体效应基因的转基因蚊子替代种群是控制疾病传播的可能方法之一。但是，具有基于单一类型效应子分子过度表达的抗病原体表型的转基因蚊子在中断病原体传播方面效率不高。在这里，我们表明两种抗微生物肽（AMP），天蚕素A和防御素A在转基因埃及伊蚊中的共过量表达导致这些AMP的协同抗菌和抗P / AS抑制作用。在卵黄原蛋白原启动子的控制下过表达Cecropin A和Defensin A的转基因杂种蚊子对铜绿假单胞菌感染的抵抗力增强，表明这些AMP协同作用于这种病原菌。在这些感染了鸡瘟假单胞菌的蚊子中，中肠的卵囊数量显着减少，当两次喂食蚊子以重新激活转基因AMP的生产时，唾液腺中没有发现子孢子。使用转基因杂交蚊子进行感染实验，然后依次喂食幼稚的鸡，然后再接种幼稚的野生型蚊子，表明疟原虫的传播被完全阻断。这项研究提出了一种产生具有抗疟原虫难治性表型的转基因蚊子的方法，该方法是两个或多个效应分子在寄生虫上的协同作用。

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  • 作者

    Vladimir Kokoza; Abdouelaziz Ahmed; Sang Woon Shin; Nwando Okafor; Zhen Zou; Alexander S. Raikhel;

  • 作者单位

    Department of Entomology and Institute for Integrative Genome Biology, University of California, Riverside, CA 92521;

    rnDepartment of Entomology and Institute for Integrative Genome Biology, University of California, Riverside, CA 92521;

    rnDepartment of Entomology and Institute for Integrative Genome Biology, University of California, Riverside, CA 92521;

    rnDepartment of Entomology and Institute for Integrative Genome Biology, University of California, Riverside, CA 92521;

    rnDepartment of Entomology and Institute for Integrative Genome Biology, University of California, Riverside, CA 92521;

    rnDepartment of Entomology and Institute for Integrative Genome Biology, University of California, Riverside, CA 92521;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    vector-borne disease; malaria; immunity; antimicrobial peptide; vitellogenin;

    机译：媒介传播疾病疟疾;免疫;抗菌肽卵黄蛋白原;

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