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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Chromosome rearrangements and aneuploidy in yeast strains lacking both Tel1p and Mec1p reflect deficiencies in two different mechanisms
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Chromosome rearrangements and aneuploidy in yeast strains lacking both Tel1p and Mec1p reflect deficiencies in two different mechanisms

机译：缺乏Tel1p和Mec1p的酵母菌株中的染色体重排和非整倍性反映了两种不同机制的缺陷

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摘要

The human ATM and ATR proteins participate in the DNA damage and DNA replication checkpoint pathways and are critical to maintaining genome stability. The Saccharomyces cerevisiae homologs of ATM and ATR are Tel1p and Mec1p, respectively. Haploid tel1 mec1 strains have very short telomeres and very high rates of chromosomal aberrations. Here, we examine genetic stability in tel1 mec1 diploid cells. In the absence of induced DNA damage, these yeast strains had very high frequencies of aneuploidy (both trisomy and monosomy) in addition to elevated rates of chromosome rearrangements. Although we found the aneuploidy in the tel1 mec1 diploids mimicked that observed in bub1 diploids, the tel1 mec1 dip-loids had a functional spindle assembly checkpoint. Restoration of wild-type telomere lengths in the tel1 mec1 strain substantially reduced the rate of chromosome rearrangements but had no effect on the frequency of aneuploidy.
机译：人类的ATM和ATR蛋白参与DNA损伤和DNA复制检查点途径，对维持基因组稳定性至关重要。 ATM和ATR的酿酒酵母同源物分别为Tel1p和Mec1p。单倍体tel1 mec1菌株具有非常短的端粒和非常高的染色体畸变率。在这里，我们检查tel1 mec1二倍体细胞的遗传稳定性。在没有诱导的DNA损伤的情况下，这些酵母菌株除了染色体重排率提高外，还具有非常高的非整倍性频率（三体性和单体性）。尽管我们发现tel1 mec1二倍体的非整倍性类似于在bub1二倍体中观察到的非整倍性，但tel1 mec1二倍体具有功能性的主轴装配检查点。恢复tel1 mec1菌株中野生型端粒的长度可显着降低染色体重排率，但对非整倍性频率没有影响。

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    Jennifer L. McCulley; Thomas D. Petes;

  • 作者单位

    Departments of Molecular Genetics and Microbiology, Duke University School of Medicine, Durham, NC 27710 Departments of Pharmacology and Cancer Biology, Duke University School of Medicine, Durham, NC 27710;

    rnDepartments of Molecular Genetics and Microbiology, Duke University School of Medicine, Durham, NC 27710 Departments of Pharmacology and Cancer Biology, Duke University School of Medicine, Durham, NC 27710;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    chromosome nondisjunction; spindle assembly checkpoint; telomeres;

    机译：染色体非分离主轴组件检查点;端粒;

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