...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Visualizing high error levels during gene expression in living bacterial cells
【24h】

Visualizing high error levels during gene expression in living bacterial cells

机译：可视化活细菌细胞中基因表达过程中的高错误水平

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

To monitor inaccuracy in gene expression in living cells, we designed an experimental system in the bacterium Bacillus subtilis whereby spontaneous errors can be visualized and quantified at a single-cell level. Our strategy was to introduce mutations into a chromosomally encoded gfp allele, such that errors in protein production are reported in real time by the formation of fluorescent GFP molecules. The data reveal that the amount of errors can greatly exceed previous estimates, and that the error rate increases dramatically at lower temperatures and during stationary phase. Furthermore, we demonstrate that when facing an antibiotic threat, an increase in error level is sufficient to allow survival of bacteria carrying a mutated antibiotic-resistance gene. We propose that bacterial gene expression is error prone, frequently yielding protein molecules that differ slightly from the sequence specified by their DNA, thus generating a cellular reservoir of nonidentical protein molecules. This variation may be a key factor in increasing bacterial fitness, expanding the capability of an isogenic population to face environmental challenges.
机译：为了监测活细胞中基因表达的不准确性，我们在枯草芽孢杆菌中设计了一个实验系统，可以在单细胞水平上可视化和量化自发错误。我们的策略是将突变引入染色体编码的gfp等位基因，从而通过荧光GFP分子的形成实时报告蛋白质生产中的错误。数据表明，误差量可能大大超过先前的估计，并且误差率在较低温度和固定阶段急剧增加。此外，我们证明当面对抗生素威胁时，错误水平的增加足以使携带突变的抗生素抗性基因的细菌存活下来。我们建议细菌基因表达容易出错，经常产生与它们的DNA指定的序列略有不同的蛋白质分子，从而产生了不同蛋白质分子的细胞库。这种变化可能是增加细菌适应性，扩大同基因种群面对环境挑战的能力的关键因素。

著录项

  • 来源
  • 作者

    Mor Meyerovich; Gideon Mamou; Sigal Ben-Yehuda;

  • 作者单位

    Department of Microbiology and Molecular Genetics, Institute for Medical Research, Israel-Canada, Hebrew University-Hadassah Medical School, Hebrew University of Jerusalem, 91120 Jerusalem, Israel;

    rnDepartment of Microbiology and Molecular Genetics, Institute for Medical Research, Israel-Canada, Hebrew University-Hadassah Medical School, Hebrew University of Jerusalem, 91120 Jerusalem, Israel;

    rnDepartment of Microbiology and Molecular Genetics, Institute for Medical Research, Israel-Canada, Hebrew University-Hadassah Medical School, Hebrew University of Jerusalem, 91120 Jerusalem, Israel;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    bacillus subtilis; translational errors; variations in living cells; translation fidelity;

    机译：枯草芽孢杆菌翻译错误;活细胞的变化;翻译保真度;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号