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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Gene targeting by homologous recombination in mouse zygotes mediated by zinc-finger nucleases
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Gene targeting by homologous recombination in mouse zygotes mediated by zinc-finger nucleases

机译：锌指核酸酶介导的小鼠受精卵中同源重组的基因靶向

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摘要

Gene targeting by homologous recombination in embryonic stem cells is extensively used to generate specific mouse mutants. However, most mammalian species lack tools for targeted gene manipulation. Since double-strand breaks strongly increase the rate of homologous recombination at genomic loci, we explored whether gene targeting can be directly performed in zygotes by the use of zinc-finger nucleases. Here we report that gene targeting is achieved in 1.7-4.5% of murine one-cell embryos upon the coinjection of targeting vectors with zinc-finger nucleases, without preselection. These findings enable the manipulation of the mammalian germ line in a single step in zygotes, independent of ES cells.
机译：通过胚胎干细胞中同源重组的基因靶向被广泛用于产生特定的小鼠突变体。然而，大多数哺乳动物物种缺乏用于靶向基因操纵的工具。由于双链断裂强烈增加了基因组位点的同源重组率，因此我们探索了是否可以通过使用锌指核酸酶直接在合子中进行基因靶向。在这里，我们报道基因靶向在与锌指核酸酶共同注射靶向载体的情况下，无需预先选择即可在1.7-4.5％的鼠单细胞胚胎中实现。这些发现使得能够在一步中在受精卵中独立于ES细胞操作哺乳动物种系。

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    Melanie Meyer; rnMartin Hrabe de Angelis; rnWolfgang Wurst; rnRalf Kuehn;

  • 作者单位

    Institute for Developmental Genetics, Helmholtz Center Munich, Munich, Germany Technical University Munich, Munich, Germany;

    rnTechnical University Munich, Munich, Germany Institute of Experimental Genetics, Helmholtz Center Munich, Munich, Germany;

    rnInstitute for Developmental Genetics, Helmholtz Center Munich, Munich, Germany Technical University Munich, Munich, Germany Deutsches Zentrum fur Neurodegenerative Erkrankungen e.V., Munich, Germany;

    rnInstitute for Developmental Genetics, Helmholtz Center Munich, Munich, Germany Technical University Munich, Munich, Germany;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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  • 正文语种 eng
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