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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Quantitative 3D elemental microtomography of Cydotella meneghiniana at 400-nm resolution
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Quantitative 3D elemental microtomography of Cydotella meneghiniana at 400-nm resolution

机译:脑膜小球藻在400 nm分辨率下的定量3D元素显微断层扫描

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摘要

X-ray fluorescence tomography promises to map elemental distributions in unstained and unfixed biological specimens in three dimensions at high resolution and sensitivity, offering unparalleled insight in medical, biological, and environmental sciences. X-ray fluorescence tomography of biological specimens has been viewed as impractical-and perhaps even impossible for routine application-due to the large time required for scanning tomography and significant radiation dose delivered to the specimen during the imaging process. Here, we demonstrate submicron resolution X-ray fluorescence tomography of a whole unstained biological specimen, quantifying three-dimensional distributions of the elements Si, P, S, Cl, K, Ca, Mn, Fe, Cu, and Zn in the freshwater diatom Cydotella meneghiniana with 400-nm resolution, improving the spatial resolution by over an order of magnitude. The resulting maps faithfully reproduce cellular structure revealing unexpected patterns that may elucidate the role of metals in diatom biology and of diatoms in global element cycles. With anticipated improvements in data acquisition and detector sensitivity, such measurements could become routine in the near future.
机译:X射线荧光层析成像技术有望以高分辨率和高灵敏度在三个维度上绘制未染色和未固定生物样品中的元素分布图,从而为医学,生物和环境科学提供无与伦比的洞察力。生物样品的X射线荧光层析成像被认为是不切实际的-甚至在常规应用中甚至是不可能的-由于扫描层析成像需要大量的时间并且在成像过程中会向样本传递大量的辐射剂量。在这里,我们展示了整个未染色生物标本的亚微米分辨率X射线荧光层析成像,量化了淡水硅藻中元素Si,P,S,Cl,K,Ca,Mn,Fe,Cu和Zn的三维分布脑膜小球藻具有400纳米的分辨率,将空间分辨率提高了一个数量级。生成的图忠实地再现了细胞结构,揭示了意想不到的模式,这些模式可能阐明了金属在硅藻生物学中的作用以及硅藻在整体元素周期中的作用。随着数据采集和检测器灵敏度的预期提高,这种测量将在不久的将来成为常规测量。

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