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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Characterization of mononuclear phagocytic cells in medaka fish transgenic for a cxcr3a:gfp reporter
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Characterization of mononuclear phagocytic cells in medaka fish transgenic for a cxcr3a:gfp reporter

机译：Cxcr3a：gfp报告基因转基因青aka鱼中单核吞噬细胞的表征

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Chemokines and chemokine receptors are key evolutionary innova-tions of vertebrates. They are involved in morphogenetic processes and play an important role in the immune system. Based on an analysis of the chemokine receptor gene family in teleost genomes, and the expression patterns of chemokine receptor genes during embryogenesis and the wounding response in young larvae of Ory-zias latipes, we identified the chemokine receptor cxcr3a as a marker of innate immune cells. Cells expressing cxcr3a were characterized in fish transgenic for a cxcr3a:gfp reporter. In embryos and larvae, cxcrJa-expressing cells are motile in healthy and damaged tissues, and phagocytic; the majority of these cells has the morphology of tissue macrophages, whereas a small fraction has a dendritic pheno-type. In adults, cxcrJa-positive cells continue to specifically express myeloid-associate markers and genes related to antigen uptake and presentation. By light microscopy and ultrastructural analysis, the majority of cxcria-expressing cells has a dendritic phenotype, whereas the remainder resembles macrophage-like cells. After chal-lenge of adult fish with bacteria or CpG oligonucleotides, phagocy-tosing cxcr3a-positive cells in the blood up-regulated H12p40 genes, compatible with their function as part of the mononuclear phago-cytic system. Our results identify a marker of teleost mononuclear phagocytic cells and suggest a surprising degree of morphological and functional similarity between the innate immune systems of lower and higher vertebrates.
机译：趋化因子和趋化因子受体是脊椎动物的关键进化创新。它们参与形态发生过程，并在免疫系统中起重要作用。通过分析硬骨鱼基因组中的趋化因子受体基因家族，以及在Ory-zias latipes幼虫胚胎发生和趋化反应过程中趋化因子受体基因的表达模式，我们确定了趋化因子受体cxcr3a是先天免疫细胞的标志物。表达cxcr3a的细胞在cxcr3a：gfp报告基因转基因鱼中进行了表征。在胚胎和幼虫中，表达cxcrJa的细胞在健康和受损的组织中能运动，并具有吞噬功能。这些细胞中的大多数具有组织巨噬细胞的形态，而一小部分具有树突状表型。在成人中，cxcrJa阳性细胞继续特异性表达与髓质相关的标志物和与抗原摄取和呈递有关的基因。通过光学显微镜和超微结构分析，大多数表达cxcria的细胞具有树突状表型，而其余的类似于巨噬细胞样细胞。在用细菌或CpG寡核苷酸挑战成年鱼后，血液中吞噬吞噬的cxcr3a阳性细胞上调了H12p40基因，使其与单核吞噬细胞系统的功能兼容。我们的结果确定了硬骨质单核吞噬细胞的标志物，并暗示了低等和高级脊椎动物先天免疫系统之间形态和功能相似性的惊人程度。

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