...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Definition of a high-affinity Gag recognition structure mediating packaging of a retroviral RNA genome
【24h】

Definition of a high-affinity Gag recognition structure mediating packaging of a retroviral RNA genome

机译：介导逆转录病毒RNA基因组包装的高亲和力Gag识别结构的定义

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

All retroviral genomic RNAs contain a cis-acting packaging signal by which dimeric genomes are selectively packaged into nascent virions. However, it is not understood how Gag (the viral structural protein) interacts with these signals to package the genome with high selectivity. We probed the structure of murine leukemia virus RNA inside virus particles using SHAPE, a high-throughput RNA structure analysis technology. These experiments showed that NC (the nucleic acid binding domain derived from Gag) binds within the virus to the sequence UCUG-UR-UCUG. Recombinant Gag and NC proteins bound to this same RNA sequence in dimeric RNA in vitro; in all cases, interactions were strongest with the first U and final G in each UCUG element. The RNA structural context is critical: High-affinity binding requires base-paired regions flanking this motif, and two UCUG-UR-UCUG motifs are specifically exposed in the viral RNA dimer. Mutating the guanosine residues in these two motifs-only four nucleotides per genomic RNA-reduced packaging 100-fold, comparable to the level of nonspecific packaging. These results thus explain the selective packaging of dimeric RNA. This paradigm has implications for RNA recognition in general, illustrating how local context and RNA structure can create information-rich recognition signals from simple single-stranded sequence elements in large RNAs.
机译：所有逆转录病毒基因组RNA均包含顺式作用包装信号，通过该信号可将二聚体基因组选择性包装到新生病毒粒子中。但是，尚不了解Gag（病毒结构蛋白）如何与这些信号相互作用以高选择性包装基因组。我们使用高通量RNA结构分析技术SHAPE探索了病毒颗粒内部的鼠白血病病毒RNA的结构。这些实验表明NC（源自Gag的核酸结合结构域）在病毒内与序列UCUG-UR-UCUG结合。重组Gag和NC蛋白在体外与二聚体RNA中的同一RNA序列结合；在所有情况下，每个UCUG元素中与第一个U和最后一个G的相互作用最强。 RNA结构的环境至关重要：高亲和力结合需要在该基序侧翼的碱基配对区域，并且两个UCUG-UR-UCUG基序专门暴露在病毒RNA二聚体中。突变这两个基序中的鸟苷残基，每个基因组RNA减少的包装只有4个核苷酸，减少了100倍，与非特异性包装的水平相当。因此，这些结果解释了二聚体RNA的选择性包装。此范例通常对RNA识别具有影响，说明了局部上下文和RNA结构如何可以从大型RNA中的简单单链序列元素创建信息丰富的识别信号。

著录项

  • 来源
  • 作者

    Cristina Gherghe; Tania Lombo; Christopher W. Leonard; Siddhartha A. K. Datta; Julian W. Bess Jr.; Robert J. Gorelick; Alan Rein; Kevin M. Weeks;

  • 作者单位

    Department of Chemistry, University of North Carolina, Chapel Hill, NC 27599-3290;

    HIV Drug Resistance Program, National Cancer Institute, Frederick, MD 21702-1201;

    Department of Chemistry, University of North Carolina, Chapel Hill, NC 27599-3290;

    HIV Drug Resistance Program, National Cancer Institute, Frederick, MD 21702-1201;

    AIDS and Cancer Virus Program, Science Applications International Corporation-Frederick, Inc., National Cancer Institute, Frederick, MD 21702-1201;

    AIDS and Cancer Virus Program, Science Applications International Corporation-Frederick, Inc., National Cancer Institute, Frederick, MD 21702-1201;

    HIV Drug Resistance Program, National Cancer Institute, Frederick, MD 21702-1201;

    Department of Chemistry, University of North Carolina, Chapel Hill, NC 27599-3290;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    retrovirus; RNA recognition code; RNA SHAPE chemistry;

    机译：逆转录病毒;RNA识别码;RNA SHAPE化学;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号