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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Transspecies dimorphic allelic lineages of the proteasome subunit β-type 8 gene (PSMB8)in the teleost genus Oryzias
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Transspecies dimorphic allelic lineages of the proteasome subunit β-type 8 gene (PSMB8)in the teleost genus Oryzias

机译：硬骨稻属蛋白酶体β亚型8型基因（PSMB8）的亚种双态等位基因谱系

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摘要

The proteasome subunit β-type 8 (PSMB8) gene in the jawed vertebrate MHC genomic region encodes a catalytic subunit of the immunoproteasome involved in the generation of peptides to be presented by'the MHC class I molecules. A teleost, the medaka [Oryzias latipes), has highly diverged dimorphic allelic lineages of the PSMB8 gene with only about 80% amino acid identity, termed "PSMB8d" and "PSMB8N," which have been retained by most wild populations analyzed. To elucidate the evolutionary origin of these two allelic lineages, seven species of the genus Oryzias were analyzed for their PSMB8 allelic sequences using a large number of individuals from wild populations. All the PSMB8 alleles of these species were classified into one of these two allelic lineages based on their nucleotide sequences of exons and introns, indicating that the Oryzias PSMB8 gene has a truly dichotomous allelic lineage. Retention of both allelic lineages was confirmed except for one species. The PSMB8d lineage showed a higher frequency than the PSMB8N lineage in all seven species. The two allelic lineages showed curious substitutions at the 31st and 53rd residues of the mature peptide, probably involved in formation of the S1 pocket, suggesting that these allelic lineages show a functional difference in cleavage specificity. These results indicate that the PSMB8 dimorphism was established before spe-ciation within the genus Oryzias and has been maintained for more than 30-60 million years under a strict and asymmetric balancing selection through several speciation events.
机译：被咬的脊椎动物MHC基因组区域中的蛋白酶体亚基8型（PSMB8）基因编码免疫蛋白酶体的催化亚基，其参与由I类MHC分子呈递的肽的产生。硬骨鱼（Medaka [Oryzias latipes]）具有高度分散的PSMB8基因双态等位基因谱系，仅具有约80％的氨基酸同一性，称为“ PSMB8d”和“ PSMB8N”，已被大多数分析的野生种群保留。为了阐明这两个等位基因谱系的进化起源，使用了来自野生种群的大量个体，分析了七属稻属的PSMB8等位基因序列。根据它们的外显子和内含子核苷酸序列，将这些物种的所有PSMB8等位基因分类为这两个等位基因谱系之一，这表明Oryzias PSMB8基因具有真正的二等分等位基因谱系。除一个物种外，两个等位基因谱系均得到保留。在所有七个物种中，PSMB8d谱系的频率均高于PSMB8N谱系。这两个等位基因谱系在成熟肽的第31位和第53位残基处显示出好奇的取代，可能与S1口袋的形成有关，表明这些等位基因谱系在切割特异性上显示出功能差异。这些结果表明，PSMB8二态性是在稻属中发生特异性之前建立的，并且通过几种物种形成事件在严格和非对称的平衡选择下已经维持了30-60百万年。

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