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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >microRNA miR-275 is indispensable for blood digestion and egg development in the mosquito Aedes aegypti
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microRNA miR-275 is indispensable for blood digestion and egg development in the mosquito Aedes aegypti

机译：microRNA miR-275对于埃及伊蚊的血液消化和卵子发育必不可少

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摘要

The mosquito Aedes aegypti is the major vector of arboviral diseases, particularly of Dengue fever, of which there are more than 100 million cases annually. Mosquitoes, such as A. aegypti, serve as vectors for disease pathogens because they require vertebrate blood for their egg production. Pathogen transmission is tightly linked to repeated cycles of obligatory blood feeding and egg maturation. Thus, the understanding of mechanisms governing egg production is necessary to develop approaches that limit the spread of mosquito-borne diseases. Previous studies have identified critical roles of hormonal- and nutrition-based target of rapa-mycin (TOR) pathways in controlling blood-meal-mediated egg maturation in mosquitoes. In this work, we uncovered another essential regulator of blood-meal-activated processes, the micro-RNA miR-275. The depletion of this microRNA in A. aegypti females after injection of its specific antagomir resulted in severe defects in blood digestion, fluid excretion, and egg development, clearly demonstrating that miR-275 is indispensable for these physiological processes. miR-275 exhibits an expression profile that suggests its regulation by a steroid hormone, 20-hydroxyec-dysone (20E). In vitro organ culture experiments demonstrated that miR-275 is induced by this hormone in the presence of amino acids, indicative of a dual regulation by 20E and TOR. This report has uncovered the critical importance of microRNAs in controlling blood-meal-activated physiological events required for completion of egg development in mosquito disease vectors.
机译：埃及伊蚊是虫媒病毒疾病（尤其是登革热）的主要媒介，每年有1亿多病例。诸如埃及伊蚊等蚊子是疾病病原体的媒介，因为它们需要脊椎动物的血液来产卵。病原体传播与强制性采血和卵成熟的重复周期紧密相关。因此，必须了解控制卵子生产的机制，以开发出限制蚊子传播疾病的方法。先前的研究已经确定了以激素和营养为基础的雷帕霉素（TOR）途径靶标在控制血液中介导的蚊子卵成熟过程中的重要作用。在这项工作中，我们发现了血粉激活过程的另一个重要调节剂，即微小RNA miR-275。注射特异的antagomir后埃及埃及雌性中此microRNA的消耗导致血液消化，体液排泄和卵子发育的严重缺陷，清楚地表明miR-275对于这些生理过程是必不可少的。 miR-275的表达谱表明其受类固醇激素20-羟基ec-dysone（20E）调控。体外器官培养实验表明，在氨基酸存在下，miR-275被这种激素诱导，表明20E和TOR双重调控。该报告揭示了微RNA在控制蚊子病媒中卵发育完成所需的血粉激活的生理事件中的至关重要性。

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