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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Low-barrier Hydrogen Bond In Photoactive Yellow Protein
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Low-barrier Hydrogen Bond In Photoactive Yellow Protein

机译：光敏黄色蛋白中的低阻隔氢键

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摘要

Low-barrier hydrogen bonds (LBHBs) have been proposed to play roles in protein functions, including enzymatic catalysis and proton transfer. Transient formation of LBHBs is expected to stabilize specific reaction intermediates. However, based on experimental results and theoretical considerations, arguments against the importance of LBHB in proteins have been raised. The discrepancy is caused by the absence of direct identification of the hydrogen atom position. Here, we show by high-resolution neutron crystallography of photoactive yellow protein (PYP) that a LBHB exists in a protein, even in the ground state. We identified 87% (819/942) of the hydrogen positions in PYP and demonstrated that the hydrogen bond between the chromophore and E46 is a LBHB. This LBHB stabilizes an isolated electric charge buried in the hydropho bic environment of the protein interior. We propose that in the excited state the fast relaxation of the LBHB into a normal hydrogen bond is the trigger for photo-signal propagation to the protein moiety. These results give insights into the novel roles of LBHBs and the mechanism of the formation of LBHBs.
机译：低屏障氢键（LBHBs）已被提议在蛋白质功能中发挥作用，包括酶催化和质子转移。 LBHBs的瞬时形成有望稳定特定的反应中间体。然而，基于实验结果和理论考虑，已经提出了反对LBHB在蛋白质中的重要性的争论。差异是由于没有直接鉴定氢原子位置引起的。在这里，我们通过光敏黄色蛋白（PYP）的高分辨率中子晶体学表明，即使在基态下，LBHB也存在于蛋白中。我们确定了PYP中87％（819/942）的氢位置，并证明了生色团和E46之间的氢键是LBHB。该LBHB稳定了埋在蛋白质内部疏水环境中的孤立电荷。我们提出，在激发态下，LBHB快速松弛成正常氢键是光信号传播到蛋白质部分的触发因素。这些结果为深入了解LBHBs的新作用以及LBHBs的形成机理提供了见识。

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  • 作者

    Shigeo Yamaguchi; Hironari Kamikubo; Kazuo Kurihara; Ryota Kuroki; Nobuo Niimura; Nobutaka Shimizu; Yoichi Yamazaki; Mikio Kataoka;

  • 作者单位

    Graduate School of Materials Science, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan;

    Graduate School of Materials Science, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan;

    Japan Atomic Energy Agency, 2-4 Shirakata-Shirane, Tokai, Ibaraki 319-1195, Japan;

    Japan Atomic Energy Agency, 2-4 Shirakata-Shirane, Tokai, Ibaraki 319-1195, Japan;

    Frontier Research Center for Applied Atomic Sciences, Ibaraki University, 4-12-1 Nakanarusawa, Hitachi, Ibaraki 316-8511, Japan;

    Japan Synchrotron Radiation Research Institute, 1-1-1 Kouto, Sayo, Hyogo, 679-5198, Japan;

    Graduate School of Materials Science, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan;

    Graduate School of Materials Science, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan Japan Atomic Energy Agency, 2-4 Shirakata-Shirane, Tokai, Ibaraki 319-1195, Japan;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    neutron crystallography; photoreaction; proton translocation; short hydrogen bond;

    机译：中子晶体学;光反应;质子易位;短氢键;

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