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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Visualizing The Protein Synthesis Machinery: New Focus On The Translational Gtpase Elongation Factor Tu
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Visualizing The Protein Synthesis Machinery: New Focus On The Translational Gtpase Elongation Factor Tu

机译：可视化蛋白质合成机制：转化Gtpase延伸因子Tu的新焦点

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摘要

Three-dimensional cryoelectron microscopy (cryo-EM) is one of the few techniques capable of visualizing large, dynamic molecules. The ribosome, a molecular machine that synthesizes proteins in the cell, provides many examples of such dynamic assemblages, and the complex between the bacterial ribosome and translation elongation factor Tu (EF-Tu), stabilized by the antibiotic kirromy-cin, was the first to be visualized by cryo-EM (1). Since that time, the technique has progressed enormously. In this issue of PNAS, Joachim Frank and colleagues report the structure of the ribosome-EF-Tu-tRNA complex at a resolution of 6.7 A (2), which presents a substantial advancement over previously reported cryo-EM reconstructions of that complex and provides new insights into its molecular architecture.rnEF-Tu is a translational GTPase that delivers aminoacyl-tRNA (aa-tRNA) to the ribosomal decoding site. Biochemical and kinetic studies have shown that the movement of aa-tRNA into the decoding site proceeds through a number of intermediate states (3). EF-Tu in the complex with aa-tRNA and GTP is recruited by ribosomal protein L12 and placed on the ribosome in such a way that the anticodon of aa-tRNA is located in the decoding site of the ribosomal 30S subunit, whereas its 3' end is bound to EF-Tu at the 50S subunit (1).
机译：三维冷冻电子显微镜（cryo-EM）是少数能够可视化大型动态分子的技术之一。核糖体是一种在细胞中合成蛋白质的分子机器，提供了许多这样的动态组装实例，而细菌核糖体和翻译延伸因子Tu（EF-Tu）之间的复合物（由抗生素切拉霉素稳定）是第一个由cryo-EM（1）可视化。从那时起，这项技术取得了巨大的进步。在本期PNAS中，约阿希姆·弗兰克（Joachim Frank）及其同事报告了核糖体-EF-Tu-tRNA复合物的结构，分辨率为6.7 A（2），这比以前报道的该复合物的冷冻-EM重建技术具有实质性的进步，并提供了rnEF-Tu是一种翻译性GTP酶，可将氨酰基tRNA（aa-tRNA）传递至核糖体解码位点。生化和动力学研究表明，aa-tRNA进入解码位点的运动通过许多中间状态进行（3）。与aa-tRNA和GTP形成复合体的EF-Tu被核糖体蛋白L12募集并放置在核糖体上，其方式是aa-tRNA的反密码子位于核糖体30S亚基的解码位点，而其3'末端在50S亚基（1）上与EF-Tu结合。

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