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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Arabidopsis 0rc1 Is A Phd-containing H3k4me3 Effector That Regulates Transcription
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Arabidopsis 0rc1 Is A Phd-containing H3k4me3 Effector That Regulates Transcription

机译：拟南芥0rc1是调节转录的含Ph的H3k4me3效应子。

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Control of gene expression depends on a complex and delicate balance of various posttranslational modifications of histones. However, the relevance of specific combinations of histone modifications is not fully defined. Downstream effector proteins recognize particular histone modifications and transduce this information into gene expression patterns. Methylation of histone H3 at lysine 4 (H3K4me) is a landmark of gene expression control in eukaryotes. Its recognition depends on the presence in the effector protein of a motif termed plant homeodomain (PHD) that specifically binds to H3K4me3. Here, we establish that Arabidopsis ORC1, the large subunit of the origin recognition complex involved in defining origins of DNA replication, functions as a transcriptional activator of a subset of genes, the promoters of which are preferentially bound by ORC1. Arabidopsis ORC1 contains a PHD and binds to H3K4me3. In addition to H4 acetylation, ORC1 binding correlates with increased H4K20me3 in the proximal promoter region of ORC1 targets. This suggests that H4K20me3, unlike in animal cells, is associated with transcriptional activation in Arabidopsis. Thus, our data provide a molecular basis for the opposite role of ORC1 in transcriptional activation in plants and repression in animals. Since only ORC1 proteins of plant species contain a PHD, we propose that plant ORC1 constitutes arnnovel class of H3K4me3 effector proteins characteristic of the plant kingdom.
机译：基因表达的控制取决于组蛋白的各种翻译后修饰的复杂而微妙的平衡。但是，组蛋白修饰的特定组合的相关性尚未完全定义。下游效应蛋白识别特定的组蛋白修饰，并将此信息转化为基因表达模式。组蛋白H3在赖氨酸4（H3K4me）处的甲基化是真核生物中基因表达控制的标志。它的识别取决于效应蛋白中是否存在与植物H3K4me3特异性结合的称为植物同源域（PHD）的基序。在这里，我们确定拟南芥ORC1是起源识别复合体的大亚基，参与定义DNA复制的起源，起着基因子集的转录激活子的作用，其启动子优先被ORC1结合。拟南芥ORC1包含PHD并与H3K4me3结合。除了H4乙酰化外，ORC1的结合还与ORC1靶标近端启动子区域中H4K20me3的增加有关。这表明与动物细胞不同，H4K20me3与拟南芥中的转录激活有关。因此，我们的数据为ORC1在植物转录激活和动物抑制中的相反作用提供了分子基础。由于仅植物物种的ORC1蛋白包含PHD，因此我们建议植物ORC1构成植物王国特有的H3K4me3效应蛋白的arnnovel类。

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