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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Following movement of the L1 stalk between three functional states in single ribosomes
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Following movement of the L1 stalk between three functional states in single ribosomes

机译：L1茎在单个核糖体的三个功能状态之间运动之后

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摘要

The L1 stalk is a mobile domain of the large ribosomal subunit E site that interacts with the elbow of deacylated tRNA during protein synthesis. Here, by using single-molecule FRET, we follow the real-time dynamics of the L1 stalk and observe its movement relative to the body of the large subunit between at least 3 distinct conformational states: open, half-closed, and fully closed. Pretrans-location ribosomes undergo spontaneous fluctuations between the open and fully closed states. In contrast, posttranslocation ribosomes containing peptidyl-tRNA and deacylated tRNA in the classical P/P and E/E states, respectively, are fixed in the half-closed conformation. In ribosomes with a vacant E site, the L1 stalk is observed either in the fully closed or fully open conformation. Several lines of evidence show that the L1 stalk can move independently of intersubunit rotation. Our findings support a model in which the mobility of the L1 stalk facilitates binding, movement, and release of deacylated tRNA by remodeling the structure of the 5QS subunit E site between 3 distinct conformations, corresponding to the E/E vacant, P/E hybrid, and classical states.
机译：L1茎是大核糖体亚基E位点的移动域，在蛋白质合成过程中与脱酰tRNA的肘部相互作用。在这里，通过使用单分子FRET，我们可以跟踪L1茎的实时动态，并观察其相对于大亚基的身体在至少3个不同的构象状态之间的运动：打开，半关闭和完全关闭。易位核糖体在开放和完全封闭状态之间经历自发波动。相反，分别在经典P / P和E / E状态下含有肽基-tRNA和脱酰基tRNA的易位核糖体被固定在半封闭构象中。在具有空缺E位点的核糖体中，以完全闭合或完全打开的构型观察到L1茎。几条证据表明，L1茎可以独立于亚基间旋转而运动。我们的发现支持一种模型，其中L1茎的活动性通过在3个不同构象之间重塑5QS亚基E位点的结构来促进脱酰tRNA的结合，运动和释放，这对应于E / E空位，P / E杂种和古典状态。

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  • 作者

    Peter V. Cornish; Dmitri N. Ermolenko; David W. Staple; Lee Hoang; Robyn P. Hickerson; Harry F. Noller; Taekjip Ha;

  • 作者单位

    Department of Physics, University of Illinois, 1110 West Green Street, Urbana, IL 61801;

    Department of Molecular, Cell, and Developmental Biology and Center for Molecular Biology of RNA, University of California, Santa Cruz, CA 95064;

    Department of Molecular, Cell, and Developmental Biology and Center for Molecular Biology of RNA, University of California, Santa Cruz, CA 95064;

    Department of Molecular, Cell, and Developmental Biology and Center for Molecular Biology of RNA, University of California, Santa Cruz, CA 95064;

    Department of Molecular, Cell, and Developmental Biology and Center for Molecular Biology of RNA, University of California, Santa Cruz, CA 95064;

    Department of Molecular, Cell, and Developmental Biology and Center for Molecular Biology of RNA, University of California, Santa Cruz, CA 95064;

    Department of Physics, University of Illinois, 1110 West Green Street, Urbana, IL 61801 Howard Hughes Medical Institute, University of Illinois, Urbana, IL 61801;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    dynamics; single-molecule FRET; translocation;

    机译：动力学;单分子FRET;易位;

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