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首页> 外文期刊>Journal of Molecular Biology >The role of L1 stalk-tRNA interaction in the ribosome elongation cycle.
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The role of L1 stalk-tRNA interaction in the ribosome elongation cycle.

机译：L1茎-tRNA相互作用在核糖体延长周期中的作用。

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摘要

The ribosomal L1 stalk is a mobile structure implicated in directing tRNA movement during translocation through the ribosome. This article investigates three aspects of L1 stalk-tRNA interaction. First, by combining data from cryo electron microscopy, X-ray crystallography, and molecular dynamics simulations through the molecular dynamics flexible fitting method, we obtained atomic models of different tRNAs occupying the hybrid P/E state interacting with the L1 stalk. These models confirm the assignment of fluorescence resonance energy transfer states from previous single-molecule investigations of L1 stalk dynamics. Second, the models reconcile how initiator tRNA(fMet) interacts less strongly with the L1 stalk compared to elongator tRNA(Phe), as seen in previous single-molecule experiments. Third, results from a simulation of the entire ribosome in which the L1 stalk is moved from a half-closed conformation to its open conformation are found to support the hypothesis that L1 stalk opening is involved in tRNA release from the ribosome.
机译：核糖体L1茎是一种可移动的结构，在通过核糖体易位期间，它指导tRNA的运动。本文研究了L1茎与tRNA相互作用的三个方面。首先，通过分子动力学灵活拟合方法，结合来自冷冻电子显微镜，X射线晶体学和分子动力学模拟的数据，我们获得了不同tRNA的原子模型，这些tRNA占据了与L1茎相互作用的杂化P / E状态。这些模型从以前对L1茎动力学的单分子研究中证实了荧光共振能量转移状态的分配。其次，与先前的单分子实验相比，该模型与启动子tRNA（Phe）相比，调和了引发剂tRNA（fMet）与L1茎的相互作用不强。第三，发现对整个核糖体的模拟结果，其中L1茎从半封闭构象移动到其开放构象，这支持L1​​茎开放参与从核糖体释放tRNA的假说。

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