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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Label-free biosensing with functionalized nanopipette probes
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Label-free biosensing with functionalized nanopipette probes

机译：带有功能化纳米吸管探针的无标记生物传感

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摘要

Nanopipette technology can uniquely identify biomolecules such as proteins based on differences in size, shape, and electrical charge. These differences are determined by the detection of changes in ionic current as the proteins interact with the nanopipette tip coated with probe molecules. Here we show that electrostatic, biotin-streptavidin, and antibody-antigen interactions on the nanopipette tip surface affect ionic current flowing through a 50-nm pore. Highly charged polymers interacting with the glass surface modulated the rectification property of the nanopipette electrode. Affinity-based binding between the probes tethered to the surface and their target proteins caused a change in the ionic current due to a partial blockade or an altered surface charge. These findings suggest that nanopipettes functionalized with appropriate molecular recognition elements can be used as nanosensors in biomedical and biological research.
机译：纳米移液技术可以根据大小，形状和电荷的差异来唯一地识别生物分子，例如蛋白质。这些差异是通过检测蛋白质与涂有探针分子的纳米移液器吸头相互作用时离子电流的变化来确定的。在这里，我们显示纳米吸管尖端表面上的静电，生物素-链霉亲和素和抗体-抗原相互作用会影响流过50 nm孔的离子电流。与玻璃表面相互作用的高电荷聚合物调节了纳米移液管电极的整流性能。拴在表面的探针与其靶蛋白之间的基于亲和力的结合会由于部分阻滞或表面电荷改变而导致离子电流发生变化。这些发现表明，用适当的分子识别元件功能化的纳米移液器可以用作生物医学和生物学研究中的纳米传感器。

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  • 作者

    Senkei Umehara; Miloslav Karhanek; Ronald W. Davis; Nader Pourmand;

  • 作者单位

    Department of Biomolecular Engineering, University of California, Santa Cruz, 1156 High Street, Santa Cruz, CA 95064 Stanford Genome Technology Center, Stanford University, 855 South California Avenue, Palo Alto, CA 94304;

    Department of Biomolecular Engineering, University of California, Santa Cruz, 1156 High Street, Santa Cruz, CA 95064;

    Stanford Genome Technology Center, Stanford University, 855 South California Avenue, Palo Alto, CA 94304;

    Department of Biomolecular Engineering, University of California, Santa Cruz, 1156 High Street, Santa Cruz, CA 95064 Stanford Genome Technology Center, Stanford University, 855 South California Avenue, Palo Alto, CA 94304;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    biomolecules; biosensor; immunoassay; current rectification; nanopore;

    机译：生物分子生物传感器免疫测定目前的整改;纳米孔;

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