Synthesis of programmable integrases

Russell M. Gordley; Charles A. Gersbach; Carlos F. Barbas Ⅲ

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Synthesis of programmable integrases

机译：可编程积分的综合

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Accurate modification of the 3 billion-base-pair human genome requires tools with exceptional sequence specificity. Here, we describe a general strategy for the design of enzymes that target a single site within the genome. We generated chimeric zinc finger recombinases with cooperative DNA-binding and catalytic specificities that integrate transgenes with >98% accuracy into the human genome. These modular recombinases can be reprogrammed: New combinations of zinc finger domains and serine recombinase catalytic domains generate novel enzymes with distinct substrate sequence specificities. Because of their accuracy and versatility, the recombi-nases/integrases reported in this work are suitable for a wide variety of applications in biological research, medicine, and biotechnology where accurate delivery of DNA is desired.

机译：准确修饰30亿个碱基对的人类基因组需要具有卓越序列特异性的工具。在这里，我们描述了设计针对基因组中单个位点的酶的一般策略。我们产生了具有合作DNA结合和催化特异性的嵌合锌指重组酶，该酶将转基因以> 98％的准确度整合到人类基因组中。这些模块重组酶可以重新编程：锌指结构域和丝氨酸重组酶催化结构域的新组合产生具有不同底物序列特异性的新型酶。由于它们的准确性和多功能性，这项工作中报道的重组/整合体适用于需要精确递送DNA的生物研究，医学和生物技术中的各种应用。

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《Proceedings of the National Academy of Sciences of the United States of America》 |2009年第13期|5053-5058|共6页
作者
Russell M. Gordley; Charles A. Gersbach; Carlos F. Barbas Ⅲ;
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作者单位

Departments of Molecular Biology and Chemistry and The Skaggs Institute for Chemical Biology, BCC 550, The Scripps Research Institute, La Jolla, CA 92037;

Departments of Molecular Biology and Chemistry and The Skaggs Institute for Chemical Biology, BCC 550, The Scripps Research Institute, La Jolla, CA 92037;

Departments of Molecular Biology and Chemistry and The Skaggs Institute for Chemical Biology, BCC 550, The Scripps Research Institute, La Jolla, CA 92037;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类
关键词
recombinases; zinc finger; gene delivery; gene targeting; protein engineering;

机译：重组酶锌指基因传递;基因靶向蛋白质工程;

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机译：设计和合成的分子探针，可调节细胞膜的渗透性：I.设计，合成和生物评价作为抗HIV-1整合酶抑制剂的新型菊苣酸类似物。二。针对膜雌激素受体的新型雌二醇类似物的设计，合成和生物学评估。
6. Synthesis of programmable integrases [O] . Russell M. Gordley, Charles A. Gersbach, Carlos F. Barbas III 2009

机译：可编程积分的综合
7. Synthesis of programmable integrases [O] . Gordley, Russell M., Gersbach, Charles A., Barbas, Carlos F. 2009

机译：可编程积分的综合

Synthesis of programmable integrases

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