...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Dicing of viral replication intermediates during silencing of latent Drosophila viruses
【24h】

Dicing of viral replication intermediates during silencing of latent Drosophila viruses

机译：果蝇潜伏病毒沉默期间病毒复制中间体的切丁

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Previous studies revealed roles for RNA interference (RNAi) in the immediate cellular response to viral infection in plants, nematodes and flies. However, little is known about how RNAi combats viruses during persistent or latent infections. Our analysis of small RNAs cloned from Drosophila cells latently infected with Flock House Virus (FHV) failed to reveal signatures of bulk degradation of the viral genome. Instead, this + strand virus specifically generated Dicer-2-dependent, 21 -nucleotide siRNAs that derived in equal proportion from + and - strands. Curiously, luciferase reporters that are fully complementary to abundant viral siRNAs were poorly repressed. Moreover, although the viral siRNAs that were incorporated into an effector complex associated with Ar-gonaute2, bulk FHV siRNAs in latently infected cells were not loaded into any Argonaute protein. Together, these data suggest that direct dicing of viral replication intermediates plays an important role in maintaining the latent viral state. In addition, the denial of bulk viral siRNAs from effector complexes suggests that criteria beyond the structural competency of RNA duplexes influence the assembly of functional silencing complexes.
机译：先前的研究揭示了RNA干扰（RNAi）在植物，线虫和果蝇对病毒感染的即时细胞反应中的作用。但是，人们对RNAi在持续或潜伏感染期间如何抵抗病毒的了解甚少。我们对从潜伏有羊群病毒（FHV）的果蝇细胞中克隆的小RNA的分析未能揭示病毒基因组大量降解的特征。相反，该+链病毒特异性生成了Dicer-2依赖性的21核苷酸siRNA，它们以等比例的方式从+和-链衍生而来。奇怪的是，与丰富的病毒siRNA完全互补的荧光素酶报道分子的抑制作用很弱。此外，尽管将病毒siRNA掺入了与Ar-gonaute2相关的效应子复合物中，但潜伏感染细胞中的大量FHV siRNA却未加载到任何Argonaute蛋白中。总之，这些数据表明病毒复制中间体的直接切割在维持潜在的病毒状态中起着重要的作用。另外，来自效应物复合物的大量病毒siRNA的拒绝表明，超出RNA双链体结构能力的标准影响功能沉默复合物的组装。

著录项

  • 来源
  • 作者

    Alex Flynt; Na Liu; Raquel Martin; Eric C. Lai;

  • 作者单位

    Department of Developmental Biology, Sloan-Kettering Institute, 1017 Rockefeller Research Laboratories, 1275 York Ave, Box 252, New York, NY 10065;

    Department of Developmental Biology, Sloan-Kettering Institute, 1017 Rockefeller Research Laboratories, 1275 York Ave, Box 252, New York, NY 10065;

    Department of Developmental Biology, Sloan-Kettering Institute, 1017 Rockefeller Research Laboratories, 1275 York Ave, Box 252, New York, NY 10065;

    Department of Developmental Biology, Sloan-Kettering Institute, 1017 Rockefeller Research Laboratories, 1275 York Ave, Box 252, New York, NY 10065;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Argonaute2; dicer-2; RNA interference; virus;

    机译：Argonaute2;dicer-2;RNA干扰;病毒;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号