...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Characterizing A Model Human Gut Microbiota Composed Of Members Of Its Two Dominant Bacterial Phyla
【24h】

Characterizing A Model Human Gut Microbiota Composed Of Members Of Its Two Dominant Bacterial Phyla

机译：表征模型的人类肠道菌群，由其两个主要细菌菌毛的成员组成

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The adult human distal gut microbial community is typically dominated by 2 bacterial phyla (divisions), the Firmicutes and the Bacteroidetes. Little is known about the factors that govern the interactions between their members. Here, we examine the niches of representatives of both phyla in vivo. Finished genome sequences were generated from Eubacterium rectale and E. eligens, which belong to Clostridium Cluster XlVa, one of the most common gut Firmicute clades. Comparison of these and 25 other gut Firmicutes and Bacteroidetes indicated that the Firmicutes possess smaller genomes and a disproportionately smaller number of glycan-degrading enzymes. Germ-free mice were then colonized with E. rectale and/or a prominent human gut Bacteroidetes, Bacteroides thetaiotaomicron, followed by whole-genome tran-scriptional profiling, high-resolution proteomic analysis, and biochemical assays of microbial-microbial and microbial-host interactions. B. thetaiotaomicron adapts to f. rectale by up-regulating expression of a variety of polysaccharide utilization loci encoding numerous glycoside hydrolases, and by signaling the host to produce mucosal glycans that it, but not E. rectale. can access. E. rectale adapts to B. thetaiotaomicron by decreasing production of its glycan-degrading enzymes, increasing expression of selected amino acid and sugar transporters, and facilitating glycolysis by reducing levels of NADH, in part via generation of butyrate from acetate, which in turn is used by the gut epithelium. This simplified model of the human gut microbiota illustrates niche specialization and functional redundancy within members of its major bacterial phyla, and the importance of host glycans as a nutrient foundation that ensures ecosystem stability.rnhuman gut Firmicutes and Bacteroidetes; carbohydrate metabolism; gnotobiotic mice; gut microbiome; nutrient sharing
机译：成年人类远端肠道微生物群落通常以2个细菌门（分裂）和拟杆菌属（Bacteroidetes）为主。关于控制其成员之间的交互的因素知之甚少。在这里，我们检查体内两种门的代表的利基。最终的基因组序列是从真细菌和大肠杆菌中产生的，它们属于梭状芽胞杆菌属XlVa，是最常见的肠纤毛分支之一。比较这些和其他25种肠道的Firmicutes和Bacteroidetes，发现这些Firmicutes具有较小的基因组和数量不成比例的较少的聚糖降解酶。然后将无胚芽小鼠定殖在直肠大肠杆菌和/或突出的人类肠道拟杆菌，拟杆菌拟杆菌中，然后进行全基因组转录谱分析，高分辨率蛋白质组学分析以及微生物-微生物和微生物宿主的生化测定互动。 B. thetaiotaomicron适应f。通过上调编码多种糖苷水解酶的多种多糖利用位点的表达，并通过发信号通知宿主产生粘膜聚糖，而不表达直肠大肠杆菌，来表达这种细菌。可以访问。直肠大肠杆菌通过减少其聚糖降解酶的产生，增加所选氨基酸和糖转运蛋白的表达，并通过降低NADH的水平来促进糖酵解而适应B. thetaiotaomicron，部分原因是由乙酸生成丁酸酯，反过来由肠上皮使用。这种简化的人类肠道菌群模型说明了其主要细菌门成员内的生态位专业化和功能冗余，以及宿主聚糖作为确保生态系统稳定性的营养基础的重要性。碳水化合物代谢gnotobiotic小鼠；肠道微生物组营养共享

著录项

  • 来源
  • 作者

    Michael A. Mahowald; Federico E. Rey; Henning Seedorf; Peter J. Turnbaugh; Robert S. Fulton; Aye Wollam; Neha Shah; Chunyan Wang; Vincent Magrini; Richard K. Wilson; Brandi L. Cantarel; Pedro M. Coutinho; Bernard Henrissat; Lara W. Crock; Alison Russell; Nathan C. Verberkmoes; Robert L. Hettich; Jeffrey I. Gordon;

  • 作者单位

    Center for Genome Sciences and Washington University School of Medicine, St. Louis, MO 63108;

    Center for Genome Sciences and Washington University School of Medicine, St. Louis, MO 63108;

    Center for Genome Sciences and Washington University School of Medicine, St. Louis, MO 63108;

    Center for Genome Sciences and Washington University School of Medicine, St. Louis, MO 63108;

    Genome Sequencing Center, Washington University School of Medicine, St. Louis, MO 63108;

    Genome Sequencing Center, Washington University School of Medicine, St. Louis, MO 63108;

    Genome Sequencing Center, Washington University School of Medicine, St. Louis, MO 63108;

    Genome Sequencing Center, Washington University School of Medicine, St. Louis, MO 63108;

    Genome Sequencing Center, Washington University School of Medicine, St. Louis, MO 63108;

    Genome Sequencing Center, Washington University School of Medicine, St. Louis, MO 63108;

    Universites Aix-Marseille I and II, Marseille, France and Unite Mixte de Recherche 6098, Marseille, France Centre National de la Recherche Scientifique, Unite Mixte de Recherche 6098, Marseille, France;

    Universites Aix-Marseille I and II, Marseille, France and Unite Mixte de Recherche 6098, Marseille, France;

    Universites Aix-Marseille I and II, Marseille, France and Unite Mixte de Recherche 6098, Marseille, France Centre National de la Recherche Scientifique, Unite Mixte de Recherche 6098, Marseille, France;

    Center for Genome Sciences and Washington University School of Medicine, St. Louis, MO 63108;

    ORNL-UTK Genome Science and Technology Graduate School, Oak Ridge National Laboratory, Oak Ridge, TN 37830;

    ORNL-UTK Genome Science and Technology Graduate School, Oak Ridge National Laboratory, Oak Ridge, TN 37830;

    ORNL-UTK Genome Science and Technology Graduate School, Oak Ridge National Laboratory, Oak Ridge, TN 37830;

    Center for Genome Sciences and Washington University School of Medicine, St. Louis, MO 63108;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号