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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Elimination of antiviral defense by viral RNase III
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Elimination of antiviral defense by viral RNase III

机译：病毒RNase III消除抗病毒防御

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摘要

Sweet potato {Ipomoea batatas) is an important subsistence and famine reserve crop grown in developing countries where Sweet potato chlorotic stunt virus (SPCSV; Closteroviridae), a single-stranded RNA (ssRNA) crinivirus, synergizes unrelated viruses in co-infected sweet potato plants. The most severe disease and yield losses are caused by co-infection with SPCSV and a potyvirus, Sweet potato feathery mottle virus (SPFMV; Potyviridae). Potyvi-ruses synergize unrelated viruses by suppression of RNA silencing with the P1/HC-Pro polyprotein; however, the SPCSV-SPFMV syn-ergism is unusual in that the potyvirus is the beneficiary. Our data show that transformation of an SPFMV-resistant sweet potato variety with the double- tranded RNA (dsRNA)-specific class 1 RNA endoribonuclease III (RNase3) of SPCSV broke down resistance to SPFMV, leading to high accumulation of SPFMV antigen and severe disease symptoms similar to the synergism in plants co-infected with SPCSV and SPFMV. RNase3-transgenic sweet potatoes also accumulated higher concentrations of 2 other unrelated viruses and developed more severe symptoms than non-transgenic plants. In leaves, RNase3 suppressed ssRNA-induced gene silencing (RNAi) in an endonuclease activity-dependent manner. It cleaved synthetic double-stranded small interfering RNAs (siRNAs) of 21, 22, and 24 bp in vitro to products of approximately 14 bp that are inactive in RNAi. It also affected total siRNA isolated from SPFMV-infected sweet potato plants, suggesting a viral mechanism for suppression of RNAi by cleavage of siRNA. Results implicate RNase3 in suppression of antiviral defense in sweet potato plants and reveal RNase3 as a protein that mediates viral synergism with several unrelated viruses, a function previously described only for P1/HC-Pro.
机译：甘薯（Ipomoea batatas）是一种重要的维持生计和饥荒的作物，在发展中国家种植，其中单链RNA（ssRNA）criminivirus甘薯褪绿特技病毒（SPCSV; Closteroviridae）可以协同感染共同感染的甘薯植物中的无关病毒。最严重的疾病和产量损失是由SPCSV和一种马铃薯病毒，甘薯羽状斑驳病毒（SPFMV; Potyviridae）共同感染引起的。马铃薯病毒通过抑制P1 / HC-Pro多蛋白对RNA的沉默来增效无关病毒。但是，SPCSV-SPFMV协同增效是不寻常的，因为波多病毒是受益者。我们的数据表明，用SPCSV的双转录RNA（dsRNA）特异的1类RNA核糖核酸酶III（RNase3）特异性的SPFMV抗性甘薯变种破坏了对SPFMV的抗性，导致SPFMV抗原的大量积累和严重疾病与SPCSV和SPFMV共同感染的植物中的增效作用相似的症状。与非转基因植物相比，RNase3转基因甘薯还积累了2种其他无关病毒的更高浓度，并出现了更严重的症状。在叶片中，RNase3以内切核酸酶活性依赖性方式抑制ssRNA诱导的基因沉默（RNAi）。它在体外将21、22和24 bp的合成双链小干扰RNA（siRNA）裂解为在RNAi中无效的大约14 bp的产物。它也影响了从受SPFMV感染的甘薯植物中分离的总siRNA，表明通过切割siRNA抑制RNAi的病毒机制。结果暗示RNase3抑制甘薯植物的抗病毒防御作用，并揭示RNase3是一种介导与几种不相关病毒协同作用的蛋白质，以前仅对P1 / HC-Pro进行了描述。

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  • 作者

    Wilmer J. Cuellar; Jan F. Kreuze; Minna-Liisa Rajamaeki; Karin R. Cruzado; Milton Untiveros; Jari P. T. Valkonen;

  • 作者单位

    Department of Applied Biology, University of Helsinki, P.O. Box 27, FIN-00014, Helsinki, Finland;

    lntegrated Crop Management Division and Germplasm Enhancement and Crop Improvement Division, International Potato Center, Apartado 1558, Lima 12, Peru Department of Plant Biology and Forest Genetics, Swedish University of Agricultural Sciences, P.O. Box 7080, SE-750 07 Uppsala, Sweden;

    Department of Applied Biology, University of Helsinki, P.O. Box 27, FIN-00014, Helsinki, Finland;

    Integrated Crop Management Division and Germplasm Enhancement and Crop Improvement Division, International Potato Center, Apartado 1558, Lima 12, Peru;

    Integrated Crop Management Division and Germplasm Enhancement and Crop Improvement Division, International Potato Center, Apartado 1558, Lima 12, Peru;

    Department of Applied Biology, University of Helsinki, P.O. Box 27, FIN-00014, Helsinki, Finland;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    plant virus; RNA silencing; suppression of RNAi; viral synergism;

    机译：植物病毒RNA沉默;抑制RNAi;病毒协同作用;

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