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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Dendro-dendritic bundling and shared synapses between gonadotropin-releasing hormone neurons
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Dendro-dendritic bundling and shared synapses between gonadotropin-releasing hormone neurons

机译：促性腺激素释放激素神经元之间的树突-树突状捆绑和共有突触

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摘要

The pulsatile release of gonadotropin-releasing hormone (GnRH) is critical for mammalian fertility, but the mechanisms underlying the synchronization of GnRH neurons are unknown. In the present study, the full extent of the GnRH neuron dendritic tree was visualized by patching and filling individual GnRH neurons with biocytin in acute brain slices from adult GnRH-green fluorescent protein (GFP) transgenic mice. Confocal analysis of 42 filled GnRH neurons from male and female adult mice revealed that the dendrites of the great majority of GnRH neurons (86%) formed multiple close appositions with dendrites of other GnRH neurons. Two types of interactions were encountered; the predominant interaction was one of vertical dendritic bundling where dendrites were found to wrap around each other in the same axis. The other interaction was one in which a GnRH neuron dendrite intercepted other GnRH neuron dendrites in a perpendicular fashion. Electron microscopy using pre-embedded, silver-enhanced immunogold labeling for both GnRH and GFP peptides in GnRH-GFP transgenic mice, confirmed that GnRH neuron dendrites were often immediately juxtaposed. Membrane specializations, including punctae and zonula adherens, were found connecting adjacent dendritic elements of GnRH neurons. Remarkably, individual afferent axon terminals were found to synapse with multiple GnRH neuron dendrites at sites of bundling. Together, these data demonstrate that GnRH neurons are not isolated from one another but, rather, interconnected via their long dendritic extensions. The observation of shared synaptic input to bundled GnRH neuron dendrites suggests a mechanism of GnRH neuron synchronization.
机译：促性腺激素释放激素（GnRH）的搏动释放对哺乳动物的生育能力至关重要，但GnRH神经元同步化的机制尚不清楚。在本研究中，通过在成年GnRH-绿色荧光蛋白（GFP）转基因小鼠的急性脑切片中用生物素修补并填充单个GnRH神经元，可以看到GnRH神经元树突树的全部范围。对来自成年和雌性成年小鼠的42个填充的GnRH神经元的共聚焦分析显示，绝大多数GnRH神经元（86％）的树突与其他GnRH神经元的树突形成多个紧密并置。遇到两种类型的交互；主要的相互作用是垂直树突状捆绑之一，其中发现树突在同一轴上彼此缠绕。另一种相互作用是GnRH神经元树突以垂直方式拦截其他GnRH神经元树突的相互作用。使用电子显微镜对GnRH-GFP转基因小鼠中的GnRH和GFP肽使用预嵌入的银增强免疫金标记，证实GnRH神经元树突通常立即并置。膜的专业化，包括点状和小带粘附，发现连接GnRH神经元的相邻树突状元素。值得注意的是，发现单个传入轴突末端在捆绑位点与多个GnRH神经元树突突触。在一起，这些数据表明GnRH神经元不是彼此隔离，而是通过其长的树突状延伸相互连接。捆绑的GnRH神经元树突共享突触输入的观察表明GnRH神经元同步化的机制。

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    Rebecca E. Campbell; Galina Gaidamaka; Seong-Kyu Han; Allan E. Herbison;

  • 作者单位

    Centre for Neuroendocrinology and Department of Physiology, University of Otago School of Medical Sciences, Dunedin, New Zealand 9054;

    Centre for Neuroendocrinology and Department of Physiology, University of Otago School of Medical Sciences, Dunedin, New Zealand 9054;

    Centre for Neuroendocrinology and Department of Physiology, University of Otago School of Medical Sciences, Dunedin, New Zealand 9054 Department of Oral Physiology, School of Dentistry, Chonbuk National University, Jeonju 561-756, South Korea;

    Centre for Neuroendocrinology and Department of Physiology, University of Otago School of Medical Sciences, Dunedin, New Zealand 9054;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    dendrite; fertility; LHRH; mouse; GnRH;

    机译：枝晶生育能力LHRH;老鼠;生殖激素;

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