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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >The bactericidal effect of a complement-independent antibody is osmolytic and specific to Borrelia
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The bactericidal effect of a complement-independent antibody is osmolytic and specific to Borrelia

机译:不依赖补体的抗体的杀菌作用是渗透性的,并且对疏螺旋体具有特异性

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摘要

A complement-independent bactericidal lgG1 against the OspB of Borrelia burgdorferi increased the permeability of the outer membrane through the creation of openings of 2.8 - 4.4 nm, resulting in its osmotic lysis. Cryo-electron microscopy and tomography demonstrated that exposure to the antibody causes the formation of outer membrane projections and large breaks which may precede the increase in permeability of the outer membrane. The bactericidal effect of this antibody is not transferable to Escherichia coli expressing rOspB on its outer membrane. Additionally, the porin P66, the only protein that coprecipitated with OspB, is dispensable for the bactericidal mechanism.
机译:通过产生2.8-4.4 nm的开口,补体无关的抗博氏疏螺旋体OspB的lgG1杀菌剂增加了外膜的通透性,从而导致其渗透裂解。低温电子显微镜和断层扫描显示,暴露于抗体会导致外膜突出物的形成和大的破裂,这可能在外膜通透性增加之前发生。该抗体的杀菌作用不可转移至在其外膜上表达rOspB的大肠杆菌。另外,孔蛋白P66是与OspB共沉淀的唯一蛋白质,对于杀菌机理是必需的。

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  • 作者单位

    Department of Molecular Genetics and Microbiology, Center for Infectious Diseases, Stony Brook University, Stony Brook, NY 11794;

    Department of Molecular Genetics and Microbiology, Center for Infectious Diseases, Stony Brook University, Stony Brook, NY 11794;

    Resource for Visualization of Biological Complexity, Wadsworth Center, New York State Department of Health, Empire State Plaza, Box 509, Albany, NY 12201-0509;

    Resource for Visualization of Biological Complexity, Wadsworth Center, New York State Department of Health, Empire State Plaza, Box 509, Albany, NY 12201-0509;

    Resource for Visualization of Biological Complexity, Wadsworth Center, New York State Department of Health, Empire State Plaza, Box 509, Albany, NY 12201-0509;

    Department of Molecular Genetics and Microbiology, Center for Infectious Diseases, Stony Brook University, Stony Brook, NY 11794;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    osmotic lysis; spirochete; outer membrane; pore; Lyme disease;

    机译:渗透裂解螺旋体外膜毛孔莱姆病;

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