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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >A luminal flavoprotein in endoplasmic reticulum-associated degradation
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A luminal flavoprotein in endoplasmic reticulum-associated degradation

机译：内质网相关降解中的腔黄素蛋白

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摘要

The quality control system of the endoplasmic reticulum (ER) discriminates between native and nonnative proteins. The latter are degraded by the ER-associated degradation (ERAD) pathway. Whereas many cytosolic and membrane components of this system are known, only few luminal players have been identified. In this study, we characterize ERFAD (ER flavoprotein associated with degradation), an ER luminal flavoprotein that functions in ERAD. Upon knockdown of ERFAD, the degradation of the ERAD model substrate ribophorin 332 is delayed, and the overall level of polyubiquitinated cellular proteins is decreased. We also identify the ERAD components SEL1L, OS-9 and ERdj5, a known reductase of ERAD substrates, as interaction partners of ERFAD. Our data show that ERFAD facilitates the dislocation of certain ERAD substrates to the cytosol, and we discuss the findings in relation to a potential redox function of the protein.
机译：内质网（ER）的质量控制系统区分天然蛋白和非天然蛋白。后者通过ER相关降解（ERAD）途径降解。尽管该系统的许多胞质和膜成分是已知的，但仅识别出很少的腔腔参与者。在这项研究中，我们表征了ERFAD（与降解相关的ER黄素蛋白），一种在ERAD中起作用的ER腔黄素蛋白。敲低ERFAD后，ERAD模型底物核糖蛋白332的降解被延迟，而多泛素化​​细胞蛋白的总体水平降低。我们还确定了ERAD组件SEL1L，OS-9和ERdj5（一种ERAD底物的已知还原酶）作为ERFAD的相互作用伙伴。我们的数据表明ERFAD有助于某些ERAD底物向细胞质中脱位，并且我们讨论了与蛋白质潜在氧化还原功能有关的发现。

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  • 作者

    Jan Riemer; Christian Appenzeller-Herzog; Linda Johansson; Bernd Bodenmiller; Rasmus Hartmann-Petersen; Lars Ellgaard;

  • 作者单位

    Department of Biology, University of Copenhagen, 2200 Copenhagen, Denmark Molecular Life Sciences, Life Science Zurich Graduate School, 8057 Zurich, Switzerland;

    Department of Biology, University of Copenhagen, 2200 Copenhagen, Denmark;

    Department of Biology, University of Copenhagen, 2200 Copenhagen, Denmark;

    Molecular Life Science Zurich Graduate School, 8057 Zurich, Switzerland Department of Molecular Systems Biology, Eidgenoessiche Technische Hochschule, 8093 Zurich, Switzerland;

    Department of Biology, University of Copenhagen, 2200 Copenhagen, Denmark;

    Department of Biology, University of Copenhagen, 2200 Copenhagen, Denmark;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    ERdj5; OS-9; SEL1L; ERFAD; ERAD;

    机译：ERdj5;OS-9;SEL1L;ERFAD;埃拉德;

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