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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >High-sensitivity microfluidic calorimeters for biological and chemical applications
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High-sensitivity microfluidic calorimeters for biological and chemical applications

机译：用于生物和化学应用的高灵敏度微流式热量计

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摘要

High-sensitivity microfluidic calorimeters raise the prospect of achieving high-throughput biochemical measurements with minimal sample consumption. However, it has been challenging to realize microchip-based calorimeters possessing both high sensitivity and precise sample-manipulation capabilities. Here, we report chip-based microfluidic calorimeters capable of characterizing the heat of reaction of 3.5-nL samples with 4.2-nW resolution. Our approach, based on a combination of hard- and soft-polymer microfluidics, provides both exceptional thermal response and the physical strength necessary to construct high-sensitivity calorimeters that can be scaled to automated, highly multiplexed array architectures. Polydimethylsiloxane microfluidic valves and pumps are interfaced to parylene channels and reaction chambers to automate the injection of analyte at 1 nL and below. We attained excellent thermal resolution via on-chip vacuum encapsulation, which provides unprecedented thermal isolation of the minute microfluidic reaction chambers. We demonstrate performance of these calorimeters by resolving measurements of the heat of reaction of urea hydrolysis and the enthalpy of mixing of water with methanol. The device structure can be adapted easily to enable a wide variety of other standard calorimeter operations; one example, a flow calorimeter, is described.
机译：高灵敏度的微流体量热仪提高了以最少的样品消耗实现高通量生化测量的前景。然而，实现同时具有高灵敏度和精确的样品处理能力的基于微芯片的量热仪具有挑战性。在这里，我们报告基于芯片的微流热量计，该热量计能够表征具有4.2nW分辨率的3.5nL样品的反应热。我们的方法基于硬聚合物和软聚合物微流体的结合，可提供出色的热响应和构造高灵敏度量热仪所需的物理强度，可将量热仪扩展到自动化的高度复用的阵列架构。聚二甲基硅氧烷微流体阀和泵连接到聚对二甲苯通道和反应室，以自动注入1 nL及以下的分析物。我们通过片上真空封装获得了出色的热分辨率，这为微小的微流控反应室提供了前所未有的热隔离。我们通过解析尿素水解反应的热量和水与甲醇混合的焓来证明这些量热仪的性能。该设备的结构可以轻松调整，以实现多种其他标准量热仪操作；描述了一个示例，流量热量计。

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