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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Single-molecule imaging reveals transforming growth factor-β-induced type II receptor dimerization
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Single-molecule imaging reveals transforming growth factor-β-induced type II receptor dimerization

机译：单分子成像显示转化生长因子-β诱导的II型受体二聚化

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摘要

Transforming growth factor-β (TGF-β) elicits its signals through two transmembrane serine/threonine kinase receptors, type II (TβRII) and type I receptors. It is generally believed that the initial receptor dimerization is an essential event for receptor activation. However, previous studies suggested that TGF-β signals by binding to the preexisting TβRII homodimer. Here, using single molecule microscopy to image green fluorescent protein (GFP)-labeled TβRII on the living cell surface, we demonstrated that the receptor could exist as monomers at the low expression level in resting cells and dimerize upon TGF-β stimulation. This work reveals a model in which the activation of serine-threonine kinase receptors is also accomplished via dimerization of monomers, suggesting that receptor dimerization is a general mechanism for ligand-induced receptor activation.
机译：转化生长因子-β（TGF-β）通过两个跨膜丝氨酸/苏氨酸激酶受体II型（TβRII）和I型受体引发其信号。通常认为，初始受体二聚化是受体活化的必要事件。但是，先前的研究表明，TGF-β通过与预先存在的TβRII同型二聚体结合而发出信号。在这里，我们使用单分子显微镜在活细胞表面上成像绿色荧光蛋白（GFP）标记的TβRII，我们证明了该受体可以以低表达水平的单体形式存在于静止细胞中，并在TGF-β刺激下二聚化。这项工作揭示了一个模型，其中丝氨酸-苏氨酸激酶受体的激活也通过单体的二聚作用来完成，这表明受体二聚作用是配体诱导的受体激活的一般机制。

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  • 作者

    Wei Zhang; Yaxin Jiang; Qiang Wang; Xinyong Ma; Zeyu Xiao; Wei Zuo; Xiaohong Fang; Ye-Guang Chen;

  • 作者单位

    Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Key Laboratory of Molecular Nanostructures and Nanotechnology, Chinese Academy of Sciences, Beijing 100190, P.R. China;

    Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Key Laboratory of Molecular Nanostructures and Nanotechnology, Chinese Academy of Sciences, Beijing 100190, P.R. China;

    State Key Laboratory of Biomembrane and Membrane Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, P. R. China;

    Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Key Laboratory of Molecular Nanostructures and Nanotechnology, Chinese Academy of Sciences, Beijing 100190, P.R. China;

    Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Key Laboratory of Molecular Nanostructures and Nanotechnology, Chinese Academy of Sciences, Beijing 100190, P.R. China;

    State Key Laboratory of Biomembrane and Membrane Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, P. R. China;

    Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Key Laboratory of Molecular Nanostructures and Nanotechnology, Chinese Academy of Sciences, Beijing 100190, P.R. China;

    State Key Laboratory of Biomembrane and Membrane Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, P. R. China;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    serine/threonine kinase receptor; subunit stoichiometry;

    机译：丝氨酸/苏氨酸激酶受体;亚基化学计量;

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