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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Mammalian MagT1 and TUSC3 are required for cellular magnesium uptake and vertebrate embryonic development
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Mammalian MagT1 and TUSC3 are required for cellular magnesium uptake and vertebrate embryonic development

机译：哺乳动物MagT1和TUSC3是细胞摄取镁和脊椎动物胚胎发育所必需的

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摘要

Magnesium (Mg~(2+)) is the second most abundant cation in cells, yet relatively few mechanisms have been identified that regulate cellular levels of this ion. The most clearly identified Mg~(2+) transporters are in bacteria and yeast. Here, we use a yeast complementary screen to identify two mammalian genes, MagT1 and TUSC3, as major mechanisms of Mg~(2+) influx. MagT1 is universally expressed in all human tissues and its expression level is up-regulated in low extracellular Mg(2+). Knockdown of either MagT1 or TUSC3 protein significantly lowers the total and free intracel-lular Mg~(2+) concentrations in mammalian cell lines. Morpholino knockdown of MagT1 and TUSC3 protein expression in zebrafish embryos results in early developmental arrest; excess Mg~(2+) or supplementation with mammalian mRNAs can rescue the effects. We conclude that MagT1 and TUSC3 are indispensable members of the vertebrate plasma membrane Mg~(2+) transport system.
机译：镁（Mg〜（2+））是细胞中含量第二高的阳离子，但是目前还没有发现调节这种离子细胞水平的机制。 Mg〜（2+）转运蛋白最清楚地存在于细菌和酵母菌中。在这里，我们使用酵母互补筛选来确定两个哺乳动物基因，MagT1和TUSC3，作为Mg〜（2+）涌入的主要机制。 MagT1在所有人类组织中普遍表达，其表达水平在低细胞外Mg（2+）中上调。敲低MagT1或TUSC3蛋白可显着降低哺乳动物细胞系中总和游离细胞内Mg〜（2+）的浓度。斑马鱼胚胎中MagT1和TUSC3蛋白表达的吗啉敲低导致早期发育停滞；过量的Mg〜（2+）或补充哺乳动物的mRNA可以挽救这种效应。我们得出结论，MagT1和TUSC3是脊椎动物质膜Mg〜（2+）传输系统必不可少的成员。

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  • 作者

    Hao Zhou; David E. Clapham;

  • 作者单位

    Department of Cardiology, Howard Hughes Medical Institute, Manton Center for Orphan Disease, Children's Hospital Boston Department of Neurobiology, Harvard Medical School, 1309 Enders, 320 Longwood Avenue, Boston, MA 02115;

    Department of Cardiology, Howard Hughes Medical Institute, Manton Center for Orphan Disease, Children's Hospital Boston Department of Neurobiology, Harvard Medical School, 1309 Enders, 320 Longwood Avenue, Boston, MA 02115;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    ALR1; transporter; TRPM; zebrafish; KMG104-AM;

    机译：ALR1;运输者TRPM;斑马鱼KMG104-AM;

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