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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Biogenesis of glutaminyl-mt tRNA~(Gln) in human mitochondria
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Biogenesis of glutaminyl-mt tRNA~(Gln) in human mitochondria

机译：人体线粒体中谷氨酰胺基mt tRNA〜（Gln）的生物发生

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摘要

Mammalian mitochondrial (mt) tRNAs, which are required for mito-chondrial protein synthesis, are all encoded in the mitochondrial genome, while mt aminoacyl-tRNA synthetases (aaRSs) are encoded in the nuclear genome. However, no mitochondrial homolog of glutaminyl-tRNA synthetase (GlnRS) has been identified in mammalian genomes, implying that Gln-tRNA~(Gln) is synthesized via an indirect pathway in the mammalian mitochondria. We demonstrate here that human mt glutamyl-tRNA synthetase (mtGluRS) efficiently misami-noacylates mt tRNA~(Gln) to form Glu-tRNA~(Gln). In addition, we have identified a human homolog of the Glu-tRNA~(Gln) amidotransferase, the hGatCAB heterotrimer. When any of the hGatCAB subunits were inactivated by siRNA-mediated knock down in human cells, the Glu-charged form of tRNA~(Gln) accumulated and defects in respiration could be observed. We successfully reconstituted in vitro Gln-tRNA~(Gln) formation catalyzed by the recombinant mtGluRS and hGatCAB. The misaminoacylated form of tRNA~(Gln) has a weak binding affinity to the mt elongation factor Tu (mtEF-Tu), indicating that the misaminoacylated form of tRNA~(Gln) is rejected from the translational apparatus to maintain the accuracy of mitochondrial protein synthesis.
机译：线粒体蛋白质合成所需的哺乳动物线粒体（mt）tRNA均在线粒体基因组中编码，而mt氨酰基tRNA合成酶（aaRSs）在核基因组中编码。然而，尚未在哺乳动物基因组中鉴定出谷氨酰胺-tRNA合成酶（GlnRS）的线粒体同源物，这暗示了Gln-tRNA-（Gln）是通过哺乳动物线粒体中的间接途径合成的。我们在这里证明了人类mt谷氨酰-tRNA合成酶（mtGluRS）有效地misami-noacylates mt tRNA〜（Gln）形成Glu-tRNA〜（Gln）。另外，我们已经鉴定出人Glu-tRNA〜（Gln）酰胺基转移酶，hGatCAB异源三聚体的同源物。当任何hGatCAB亚基被siRNA介导的敲除在人细胞中失活时，tRNA〜（Gln）的Glu电荷形式就会积累，并观察到呼吸缺陷。我们成功地重组了mtGluRS和hGatCAB催化了体外Gln-tRNA〜（Gln）的形成。 tRNA〜（Gln）的氨基酰化形式与mt延伸因子Tu（mtEF-Tu）的结合亲和力较弱，表明tRNA〜（Gln）的氨基化形式被翻译装置拒绝，以维持线粒体蛋白的准确性合成。

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  • 作者

    Asuteka Nagao; Takeo Suzuki; Takayuki Katoh; Yuriko Sakaguchi; Tsutomu Suzuki;

  • 作者单位

    Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Building 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan;

    Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Building 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan;

    Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Building 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan;

    Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Building 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan;

    Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Building 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    EF-Tu; Glu-tRNAGln amidotransferase; glutamyl-tRNA synthetase; mitochondrial tRNA;

    机译：EF-Tu;Glu-tRNAGln酰胺转移酶;谷氨酰-tRNA合成酶;线粒体tRNA;

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