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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Identification of functional marker proteins in the mammalian growth cone
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Identification of functional marker proteins in the mammalian growth cone

机译:哺乳动物生长锥中功能标记蛋白的鉴定

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摘要

Identification of proteins in the mammalian growth cone has the potential to advance our understanding of this critical regulator of neuronal growth and formation of neural circuit; however, to date, only one growth cone marker protein, GAP-43, has been reported. Here, we successfully used a proteomic approach to identify 945 proteins present in developing rat forebrain growth cones, including highly abundant, membrane-associated and actin-associated proteins. Almost 100 of the proteins appear to be highly enriched in the growth cone, as determined by quantitative immunostaining, and for 17 proteins, the results of RNAi suggest a role in axon growth. Most of the proteins we identified have not previously been implicated in axon growth and thus their identification presents a significant step forward, providing marker proteins and candidate neuronal growth-associated proteins.
机译:鉴定哺乳动物生长锥中的蛋白质有可能加深我们对这种神经元生长和神经回路形成的关键调节因子的理解。然而,迄今为止,仅报道了一种生长锥标记蛋白GAP-43。在这里,我们成功地使用了蛋白质组学方法来鉴定存在于发育中的大鼠前脑生长锥中的945种蛋白质,包括高度丰富的,膜相关的和肌动蛋白相关的蛋白质。通过定量免疫染色确定,几乎100种蛋白质似乎在生长锥中高度富集,而对于17种蛋白质,RNAi的结果表明在轴突生长中起作用。我们鉴定出的大多数蛋白质以前都没有参与轴突的生长,因此它们的鉴定代表了向前迈出的重要一步,可提供标记蛋白和候选神经元生长相关蛋白。

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  • 作者单位

    Divisions of Molecular Cell Biology, Niigata University, 1-757 Asahi-machi, Chuo-ku, Niigata, Niigata 951-8510, Japan Divisions of Transdisciplinary Research Program, Niigata University, 1-757 Asahi-machi, Chuo-ku, Niigata, Niigata 951-8510, Japan;

    Divisions of Molecular Cell Biology, Niigata University, 1-757 Asahi-machi, Chuo-ku, Niigata, Niigata 951-8510, Japan Divisions of Ophthalmology, Graduate School of Medical and Dental Sciences, Niigata University, 1-757 Asahi-machi, Chuo-ku, Niigata, Niigata 951-8510, Japan;

    Divisions of Molecular Cell Biology, Niigata University, 1-757 Asahi-machi, Chuo-ku, Niigata, Niigata 951-8510, Japan;

    Divisions of Molecular Cell Biology, Niigata University, 1-757 Asahi-machi, Chuo-ku, Niigata, Niigata 951-8510, Japan Divisions of Ophthalmology, Graduate School of Medical and Dental Sciences, Niigata University, 1-757 Asahi-machi, Chuo-ku, Niigata, Niigata 951-8510, Japan;

    Divisions of Molecular Cell Biology, Niigata University, 1-757 Asahi-machi, Chuo-ku, Niigata, Niigata 951-8510, Japan Divisions of Transdisciplinary Research Program, Niigata University, 1-757 Asahi-machi, Chuo-ku, Niigata, Niigata 951-8510, Japan;

    Department of Chemistry, Graduate School of Science, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji, Tokyo 192-0397, Japan Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Sanbancho 5, Chiyoda-ku, Tokyo 102-0075, Japan;

    Department of Chemistry, Graduate School of Science, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji, Tokyo 192-0397, Japan Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Sanbancho 5, Chiyoda-ku, Tokyo 102-0075, Japan;

    Chiba Industry Advancement Center , Kazusa DNA Research Institute, 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba 292-0818, Japan Collaboration of Regional Entities for the Advancement of Technological Excellence Program, Kazusa DNA Research Institute, 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba 292-0818, Japan;

    Divisions of Molecular Cell Biology, Niigata University, 1-757 Asahi-machi, Chuo-ku, Niigata, Niigata 951-8510, Japan Divisions of Transdisciplinary Research Program, Niigata University, 1-757 Asahi-machi, Chuo-ku, Niigata, Niigata 951-8510, Japan;

    Department of Chemistry, Graduate School of Science, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji, Tokyo 192-0397, Japan Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Sanbancho 5, Chiyoda-ku, Tokyo 102-0075, Japan;

    Divisions of Molecular Cell Biology, Niigata University, 1-757 Asahi-machi, Chuo-ku, Niigata, Niigata 951-8510, Japan Divisions of Transdisciplinary Research Program, Niigata University, 1-757 Asahi-machi, Chuo-ku, Niigata, Niigata 951-8510, Japan;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    GAP-43; proteomics; RNAi; neuronal growth-associated proteins; axon guidance;

    机译:GAP-43;蛋白质组学RNAi;神经元生长相关蛋白;轴突指导;

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