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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Visually evoked activity in cortical cells imaged in freely moving animals
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Visually evoked activity in cortical cells imaged in freely moving animals

机译：自由活动的动物在皮层细胞中视觉诱发的活动

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摘要

We describe a miniaturized head-mounted multiphoton microscope and its use for recording Ca~(2+) transients from the somata of layer 2/3 neurons in the visual cortex of awake, freely moving rats. Images contained up to 20 neurons and were stable enough to record continuously for >5 min per trial and 20 trials per imaging session, even as the animal was running at velocities of up to 0.6 m/s. Neuronal Ca~(2+) transients were readily detected, and responses to various static visual stimuli were observed during free movement on a running track. Neuronal activity was sparse and increased when the animal swept its gaze across a visual stimulus. Neurons showing preferential activation by specific stimuli were observed in freely moving animals. These results demonstrate that the multiphoton fiberscope is suitable for functional imaging in awake and freely moving animals.
机译：我们描述了一个小型的头戴式多光子显微镜及其在从清醒，自由移动的大鼠的视觉皮层中记录来自2/3层神经元的躯体的Ca〜（2+）瞬变的用途。图像包含多达20个神经元，并且即使在动物以高达0.6 m / s的速度运行时，其稳定性也足以连续记录每次试验> 5分钟和每次成像会话20次。神经元Ca〜（2+）瞬变很容易被检测到，并且在运行轨迹上自由运动期间观察到对各种静态视觉刺激的响应。当动物通过视觉刺激扫视时，神经元活动稀疏并增加。在自由移动的动物中观察到神经元显示出受到特定刺激的优先激活。这些结果表明，多光子纤维镜适用于清醒和自由移动的动物的功能成像。

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